(2-Hydroxyethyl)dimethyl(3-sulfopropyl)ammonium Hydroxide Inner Salt [for Biochemical Research] TCI H1399

(2-Hydroxyethyl)dimethyl(3-sulfopropyl)ammonium Hydroxide Inner Salt is a zwitterionic sulfobetaine compound prepared specifically for biochemical research work. Within a single molecule it carries both a positively charged quaternary ammonium group and a negatively charged sulfonate group, so the compound is overall neutral in character yet strongly polar. The additional hydroxyethyl group further improves its solubility in water. In the laboratory, additives of this type are used to help keep proteins in solution, to reduce aggregation during purification, and to improve sample behaviour across a range of charge-based separation techniques.

The principal advantage of zwitterionic compounds in the sulfobetaine class is that they carry no net charge across the pH range normally used in biochemical work, so adding them does not interfere with charge-based separations such as ion-exchange chromatography or isoelectric focusing. Unlike strongly ionic detergents, which readily denature biological material, zwitterionic additives generally act more gently on protein structure. Their highly hydrophilic nature means they dissolve easily in aqueous buffers and can be removed again without difficulty by dialysis or ultrafiltration. This combination of properties makes the compound a practical choice where a solubilising additive is needed but charge neutrality and mildness must be preserved.

In Indonesian laboratories, materials of this kind are typically found in university biochemistry and molecular biology facilities, in research institute protein laboratories, and in analytical groups working on separation methods. They are handled as a research-grade buffer additive rather than as a bulk process chemical, generally weighed out in small quantities and prepared as stock solutions in aqueous buffer immediately before use. The material is supplied under the TCI brand and is intended for research applications rather than clinical, diagnostic, or therapeutic use.

• Protein solubilisation during extraction and purification, where the zwitterionic sulfobetaine structure helps keep target proteins in solution without introducing the net charge that would disturb downstream handling steps.
• Suppression of protein aggregation in concentrated samples, since the strongly polar but charge-neutral molecule interacts with protein surfaces gently and reduces the tendency of material to precipitate out.
• Ion-exchange chromatography sample preparation, because the additive carries no net charge at normal biochemical working pH and therefore does not compete with or distort the charge-based separation mechanism.
• Isoelectric focusing buffer formulation, where a charge-neutral solubilising additive is required so that the pH gradient and the migration of proteins to their isoelectric points remain undisturbed.
• General buffer additive work in biochemical research, taking advantage of high water solubility for easy dissolution and straightforward removal afterwards by dialysis or ultrafiltration.

• Grade: labelled by the manufacturer for biochemical research use

Store the container tightly closed in a cool, dry place away from direct sunlight and moisture, following the storage conditions printed on the manufacturer's label. Because the material is highly hydrophilic, keep the original container well sealed and avoid prolonged exposure to humid air; glass or chemically compatible plastic containers with tight closures are suitable for any prepared stock solutions. Handle the solid in a well-ventilated area or fume hood, wear a laboratory coat, safety glasses, and suitable gloves, and avoid generating dust during weighing. Use clean, dry spatulas to prevent contamination of the stock material. Prepared aqueous solutions should be clearly labelled with contents and preparation date. Consult the manufacturer's safety data sheet before first use and dispose of waste in accordance with applicable laboratory and local regulations.

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