N-(1-Naphthyl)ethylenediamine Dihydrochloride [for Biochemical Research] TCI N0869

N-(1-Naphthyl)ethylenediamine Dihydrochloride [for Biochemical Research], TCI product code N0869, is the dihydrochloride salt of a naphthylamine derivative that carries an ethylenediamine chain. It is often abbreviated as NED. In the laboratory it is best known as the key component of the Griess reagent, which is used to determine nitrite ions by colorimetry or spectrophotometry. The "for Biochemical Research" grade means it is suited to analytical and biochemical work where results must be consistent and repeatable.

Chemists choose this material because it reacts with diazonium salts to form a strongly colored, stable azo dye. In the Griess method, nitrite first diazotizes an aromatic amine such as sulfanilamide. The diazonium salt formed in that step is then coupled with NED, giving a color whose intensity is proportional to the nitrite concentration. Compared with other coupling reagents, NED forms a soluble product and gives good sensitivity. The dihydrochloride salt form also dissolves easily in water or acidic solutions, which makes reagent preparation simpler and more reliable.

In Indonesia, NED is widely needed by environmental laboratories that analyze nitrite and nitrate in water samples. Food laboratories use it to test for nitrite, and research and academic laboratories use it for colorimetric assays that need a dependable coupling agent. Its long history in standardized Griess-based procedures makes it a familiar reagent for routine monitoring, method validation, teaching practicals, and quality control work in both public and private laboratories.

• Nitrite determination in water: NED is the coupling component of the Griess reagent and produces a stable azo color that can be measured by spectrophotometry.
• Nitrate analysis after reduction: once nitrate is reduced to nitrite, the same NED-based Griess reaction gives a sensitive, proportional color response for quantification.
• Food testing for nitrite: food laboratories use NED to check nitrite in samples because the soluble azo product allows clear and repeatable colorimetric readings.
• Biochemical and research assays: the biochemical research grade supports experiments that measure nitrite as a reaction product and need consistent, reproducible color development.
• Teaching and method validation: its well-established diazotization and coupling chemistry makes NED a practical reagent for demonstrating and validating classic colorimetric analytical methods.

• Grade: for Biochemical Research

Keep N-(1-Naphthyl)ethylenediamine Dihydrochloride in its original, tightly sealed container in a cool, dry, well-ventilated area, away from moisture, direct light, and incompatible materials such as strong oxidizing agents. Close the container promptly after each use so the solid does not absorb humidity. Prepared Griess reagent solutions are best stored in clean, labeled glass bottles and made fresh as required by the analytical method. Wear gloves, safety glasses, and a lab coat when handling the material. Avoid creating or breathing dust, weigh it in a well-ventilated area or under a fume hood, and wash hands after use. Always read the Safety Data Sheet supplied with the product before use, and dispose of waste according to local regulations.

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