TCI

Protein A Agarose TCI P2461

Chemical Identity

PubChem: SID 354334260

Rp 6.811.350,00

~~Rp 4.778.550,00~~ Rp 6.811.350,00

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TCI P2461 Protein A Agarose is an affinity resin made of Protein A immobilized on agarose beads. Protein A comes from the bacterium *Staphylococcus aureus* and binds the Fc region of immunoglobulin G (IgG) from many species. Laboratories use this resin to purify antibodies, perform immunoprecipitation, and capture immune complexes from biological samples such as serum, cell culture supernatants, and lysates. This makes it a core tool for antibody-focused work in chemical biology and glycoscience.

Researchers choose this resin because Protein A binds the antibody Fc region specifically and reversibly. Antibodies bind under neutral conditions and can then be released by changing the buffer, usually with a low-pH elution buffer. The agarose matrix is hydrophilic and has wide pores, so large proteins can reach the binding sites easily and non-specific binding stays low. Because Protein A binds at the Fc region, the antigen-binding sites of the captured antibody usually remain free. This is a clear advantage in immunoprecipitation, where the bound antibody still has to recognize and pull down its target antigen.

In Indonesia, Protein A Agarose is relevant to molecular biology, immunology, biotechnology, and biochemistry laboratories at universities, teaching hospitals, and research institutions. Research groups use it to purify antibodies from serum or hybridoma culture supernatants, to isolate target proteins through immunoprecipitation, and to prepare samples for downstream analyses such as electrophoresis and Western blotting. It suits both routine teaching work and more advanced research projects that need dependable, reproducible antibody capture.

Advantages (TCI brochure)

Protein A mutant allows antibody elution under mild conditions (pH 4.0)
Most antibodies do not denature at pH 4.0 elution
Protein A covalently coupled to agarose resin

TCI brochures (PDF)

Antibodies and Related Reagents 2026-07-29 · p. 9
Reagents for Glyco Chemistry & Biology 5th Edition · p. 182
Reagents for Biological Research 2025-03-11 · p. 15

Related TCI products

Antibody purification: Protein A binds the IgG Fc region specifically, so IgG can be captured from serum or culture supernatants and then eluted with a low-pH buffer.
Immunoprecipitation: The Fc-directed binding leaves the antigen-binding sites free, so antibodies on the beads can still capture their target proteins from cell or tissue lysates.
Co-immunoprecipitation of protein complexes: The hydrophilic, wide-pore agarose matrix keeps non-specific binding low, which helps separate genuine interaction partners from background proteins.
Immune complex capture: The resin binds antibody-antigen complexes through the Fc region, so researchers can isolate and study immune complexes in biological samples.
Sample preparation for downstream analysis: Proteins captured on the resin can be eluted and analyzed by electrophoresis, Western blotting, or other protein characterization methods.

From the TCI brochure

Antibody purification
Immunoprecipitation

Brand	TCI
CAS number	—
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Glycoscience
Pack sizes	as listed on the product page
Physical form	—
Storage	follow the conditions on the product label and Safety Data Sheet

Product code: P2461
Composition: Protein A from *Staphylococcus aureus* immobilized on agarose beads

Data from the TCI brochure

Elution pH	pH 4.0 (majority of applied human IgG eluted) (Purification of human IgG, Figure 1)

Source: TCI brochure L3026 (2026-07-29)

Store Protein A Agarose under the conditions shown on the product label and Safety Data Sheet. Biological affinity resins like this one are generally kept refrigerated and must not be frozen, because freezing can damage the agarose bead structure. Keep the resin in its original, tightly closed container and prevent it from drying out. Mix the slurry gently before use so the beads are evenly suspended, and use clean pipettes and buffers to avoid microbial or protein contamination. Wear gloves, a laboratory coat, and eye protection when working with the resin and its buffers. Handle all biological samples according to your institution's biosafety procedures, and dispose of used resin and waste solutions in line with local laboratory regulations.

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