• TCI P3291 Polyethylene Glycol Mono-4-octylphenyl Ether (n=approx. 10) Solution (10w/v% in H2O) [for Molecular Biology]
  • Polyethylene Glycol Mono-4-octylphenyl Ether (n=approx. 10) Solution (10w/v% in H2O) [for Molecular Biology] - AMI Scientific
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TCI

Polyethylene Glycol Mono-4-octylphenyl Ether (n=approx. 10) Solution (10w/v% in H2O) [for Molecular Biology] TCI P3291

Polyethylene Glycol Mono-4-octylphenyl Ether (n=approx. 10) Solution (10w/v% in H2O) [for Molecular Biology] TCI P3291
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Polyethylene Glycol Mono-4-octylphenyl Ether (n = approx. 10) is a nonionic detergent from the octylphenol ethoxylate family. Its molecule has a hydrophobic octylphenyl group and a hydrophilic polyethylene glycol chain of about ten ethylene oxide units. This TCI product comes as a ready-to-use 10 w/v% solution in water and is intended for molecular biology. In biochemistry laboratories, detergents of this type are used to lyse cell membranes, solubilize membrane proteins and reduce nonspecific binding in many assays.

Nonionic detergents are chosen because they are relatively gentle on proteins. Ionic detergents such as SDS tend to denature proteins. Octylphenol ethoxylates, by contrast, can usually disrupt the lipid layer of a membrane while keeping the structure and activity of many proteins intact. The 10% solution format is very practical. The neat detergent is viscous and hard to pipette accurately, but this ready-made solution is easy to dilute to the much lower concentrations normally used in experiments. The "for Molecular Biology" grade means the product is prepared for molecular biology applications that need consistent results.

In Indonesian molecular biology and biochemistry laboratories, this detergent solution is commonly found in university research groups, hospital and diagnostic laboratories, and biotechnology research institutes. Researchers add it to cell lysis buffers, protein extraction protocols, and the wash or blocking buffers used in immunoassays. Because it is supplied as a ready-to-use aqueous solution, preparing working buffers is faster and more reproducible, which is useful in teaching laboratories and in research groups that run routine experiments.

Related TCI products

  • Cell lysis: the nonionic detergent breaks down the lipid bilayer of cell membranes, releasing cellular contents while keeping many proteins in a native, active state for later analysis.
  • Membrane protein solubilization: the amphiphilic structure surrounds hydrophobic regions of membrane proteins and brings them into aqueous solution without the strong denaturing effect of ionic detergents.
  • Reducing nonspecific binding in immunoassays: at low concentrations in wash and blocking buffers it helps cut background signal in techniques such as ELISA and Western blotting.
  • Molecular biology buffer preparation: the 10 w/v% aqueous format allows accurate, quick dilution into lysis, extraction and reaction buffers, avoiding the difficulty of pipetting viscous neat detergent.
  • Extraction of enzymes and proteins for activity studies: its gentle action on protein structure makes it suitable when enzymatic activity or protein function must be kept after extraction.

Brand TCI (product code P3291)
CAS number —
Molecular formula —
Purity —
Category Life Science > Biochemicals and Reagents > Detergents (for Biochemistry)
Pack sizes see the product listing and the manufacturer's label or SDS
Physical form Solution, 10 w/v% in H2O
Storage —
  • Product: Polyethylene Glycol Mono-4-octylphenyl Ether (n = approx. 10), nonionic octylphenol ethoxylate detergent
  • Grade: for Molecular Biology

Keep the solution in its original, tightly closed container. Store it in a clean, cool and dry place away from direct sunlight, following the storage conditions on the TCI label and safety data sheet. To protect molecular biology work, use clean, nuclease-free pipette tips and tubes when you take out portions, and do not return unused solution to the original bottle. Mix gently before use to avoid heavy foaming. Wear standard laboratory protective equipment, including gloves, safety glasses and a lab coat, and avoid contact with eyes and skin. Dispose of waste solutions according to your institution's laboratory chemical waste procedures and local regulations.

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