Highly Selective GSK-3 Inhibitor for iPS Cell Reprogramming and Organoid Culture

CHIR 99021 [C4010] is a small-molecule inhibitor with high specificity for glycogen synthase kinase 3 (GSK-3), available in a cell culture grade that has been tested for endotoxin and mycoplasma contamination. GSK-3 regulates multiple signaling pathways in a context-dependent manner, including glycogen and protein synthesis, cell death and survival,¹) mRNA methylation,²) differentiation within the colonic secretory niche,³) and cell proliferation via the canonical Wnt pathway. Inhibition of GSK-3 stabilizes β-catenin, activating Wnt target genes and promoting long-term self-renewal. This involves suppression of Tcf3, a transcriptional repressor of pluripotency factors such as Oct4, Sox2, and Nanog.⁴,⁵) Accordingly, CHIR 99021 is widely used in chemical reprogramming cocktails for iPSC generation and in organoid culture systems where Wnt activation is required.

Related products

• TCI C2943 252917-06-9 CHIR 99021
• TCI A3324 909910-43-6 A 83-01
• TCI R0224 446859-33-2 RepSox
• TCI G0639 700874-72-2 Galunisertib

References

1. Identification of an antiapoptotic protein complex at death receptors
2. M. Sun, L. Song, Y. Li, T. Zhou, R. S. Jope, Cell Death Differ. 2008, 15, 1887.
3. Glycogen synthase kinase-3 (GSK-3) activity regulates mRNA methylation in mouse embryonic stem cells
4. K. J. Faulds, J. N. Egelston, L. J. Sedivy, M. K. Mitchell, S. Garimella, H. Kozlowski, A. D’Alessandro, K. C. Hansen, J. L. Balsbaugh, C. J. Phiel, J. Biol. Chem. 2018, 293, 10731.
5. Reciprocal targeting of Hath1 and β-catenin by Wnt glycogen synthase kinase 3β in human colon cancer
6. K. Tsuchiya, T. Nakamura, R. Okamoto, T. Kanai, M. Watanabe, Gastroenterology 2007, 132, 208.
7. Wnt Pathway Regulation of Embryonic Stem Cell Self-Renewal
8. B. J. Merrill, Cold Spring Harb. Perspect. Biol. 2012, 4, a007971.
9. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation
10. J. Wray, T. Kalkan, S. Gomez-Lopez, D. Eckardt, A. Cook, R. Kemler, A. Smith, Nat. Cell Biol. 2011, 13, 838.
11. << Prev
12. Next >>
13. Page Top

Source

• TCI Product Highlights: www.tcichemicals.com/ID/en/product/tci-topics/ProductHighlights_20260525
• TCI product page C2943: tcichemicals.com/ID/en/p/C2943

Ask AMI via WhatsApp

For research and laboratory use only, not for human consumption.