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Description
TCI C2943 is CHIR 99021, a small-molecule compound widely recognised as an inhibitor of glycogen synthase kinase-3 (GSK-3) and extensively used to chemically activate the Wnt/β-catenin signalling pathway. In cell biology laboratories, this compound serves as a component of culture medium supplements that keep stem cells and progenitor cells in an undifferentiated state, while at the same time promoting the formation and expansion of three-dimensional organoid structures. Its availability allows researchers to direct cell fate without resorting to genetic modification, making culture systems simpler, more reproducible, and easier to compare between laboratories.
The principal characteristic that makes CHIR 99021 a preferred choice is its high selectivity for GSK-3 relative to many other kinases, so that effects observed in culture can be interpreted more confidently as a consequence of Wnt pathway activation rather than off-target inhibition. The compound is also active at low working concentrations, dissolves well in organic solvents such as dimethyl sulfoxide and can therefore be prepared conveniently as a concentrated stock solution, and acts reversibly, allowing researchers to withdraw the stimulus at any point in a protocol. Together these properties give a level of experimental control that is difficult to achieve with genetic approaches.
In Indonesian laboratories, CHIR 99021 is typically found in university research groups, medical faculties, and biomedical research institutes that maintain stem cell and organoid culture programmes. It is generally used as part of a defined supplement package added to base media, prepared once as a stock solution in dimethyl sulfoxide and then aliquoted for repeated experiments. Because it is a small-molecule reagent used at low concentrations, a single package usually supports an extended series of culture runs across a research programme.
Laboratory Applications
Organoid culture establishment and expansion — chemical activation of Wnt/β-catenin signalling supports the formation and continued propagation of three-dimensional organoid structures without genetic manipulation of the source cells.
Stem cell maintenance in the undifferentiated state — as a medium supplement component, it helps keep stem and progenitor populations from spontaneously differentiating during routine passaging and expansion.
Directed differentiation protocols — because inhibition is reversible, researchers can apply and then withdraw Wnt pathway stimulation at defined time points to guide cells along a chosen developmental route.
GSK-3 signalling pathway studies — its high selectivity for GSK-3 over many other kinases makes observed cellular responses easier to attribute specifically to Wnt/β-catenin pathway activation.
Reproducible and transferable culture system development — a defined small-molecule additive at low working concentration simplifies protocols and makes culture conditions easier to compare between different laboratories.
Specifications
Brand: TCI
CAS number: 252917-06-9
Category: Life Science > Cell Culture > Organoid Culture
Solubility: soluble in organic solvents such as dimethyl sulfoxide (DMSO), suitable for concentrated stock preparation
Pack sizes and storage: refer to the manufacturer's packaging and product documentation for the available pack configurations and the specified storage temperature
Handling and Storage
Store the compound according to the conditions stated by the manufacturer on the product label and documentation, keeping the container tightly closed and protected from moisture. Stock solutions prepared in dimethyl sulfoxide should be divided into small single-use aliquots in tightly sealed, solvent-compatible vials to avoid repeated freeze-thaw cycles that may reduce activity. Weighing and solution preparation are best carried out in a chemical fume hood or a suitable enclosure, with laboratory coat, gloves, and eye protection worn throughout. Additions to culture medium should be made under aseptic conditions in a biological safety cabinet. Label all aliquots clearly with the compound name, concentration, solvent, and preparation date, and dispose of unused material and contaminated consumables in accordance with institutional chemical waste procedures.
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