Collection: Nucleobase-specific Probes [RNA Structure Analysis]

Nucleobase-specific Probes [RNA Structure Analysis] — includes chemical reagents that react selectively with certain nitrogen bases in nucleic acids, such as alkylating agents and carbodiimide-based reagents. This group serves as chemical probes for mapping the secondary structure and base accessibility of RNA molecules.

Diethyl pyrocarbonate and dimethyl sulfate are used in RNA structure mapping techniques based on chemical modification (chemical probing), such as SHAPE or DMS-MaPseq, to identify RNA regions that are paired or unpaired. Carbodiimide reagents are used for modifying uracil and guanine bases in studies of RNA-protein interactions. Molecular biology and RNA structure researchers use these probes in transcriptome analysis and RNA fold mapping both in vitro and in vivo.

Example products in this collection: TCI O0103 1609-47-8 Diethyl Pyrocarbonate, TCI C0793 2491-17-0 1-Cyclohexyl-3-(2-morpholinoethyl)carbodiimide Metho-p-toluenesulfonate [for Peptide Synthesis], TCI N1268 4013-72-3 Nicotinoyl Azide, TCI D0797 77-78-1 Dimethyl Sulfate.

Note the stability of these reagents, which are generally reactive and sensitive to moisture, so storage and handling under water-free conditions is important before use in modification reactions. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other categories at the same level under RNA Structure Analysis, often used together in one laboratory workflow:

• SHAPE Probes [RNA Structure Analysis]
• Sulfonylation Probe [RNA Structure Analysis]

Back to RNA Structure Analysis. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — official distributor of TCI Japan in Indonesia — to check availability and lead time directly from Japan.