2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate [for HPLC Labeling] TCI A5514

Chemical Identity

Other names: GITC
CAS No.: 14152-97-7
PubChem: CID 99462·SID 87563161
Formula: C15H19NO9S
Molecular weight: 389.4 g/mol
Purity: >98.0%(HPLC)(N)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: [(2R,3R,4S,5R,6R)-3,4,5-triacetyloxy-6-isothiocyanatooxan-2-yl]methyl acetate
SMILES: CC(=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)N=C=S)OC(=O)C)OC(=O)C)OC(=O)C
InChIKey: WOWNQYXIQWQJRJ-UXXRCYHCSA-N

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TCI A5514, also known as 2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate, is a specialized chemical compound used in chemical biology and glycoscience research. This compound serves as a labeling agent for High-Performance Liquid Chromatography (HPLC), enabling scientists to track and identify complex molecules within mixtures. Its role in laboratory settings is crucial for analyzing the structure and function of glycans, which are essential in biological processes. The compound’s unique chemical structure allows it to interact specifically with target molecules, making it a valuable tool in analytical chemistry.

The compound’s preferred status stems from its high reactivity and specificity, which enhance the accuracy and efficiency of labeling processes. The isothiocyanate group acts as a labeling moiety, ensuring strong and stable attachment to biomolecules. Its chemical properties allow for selective reactions, reducing background noise and improving detection limits. These characteristics make it ideal for applications requiring high precision and reliability, such as in drug development and diagnostic research. The compound’s stability under controlled conditions further supports its use in various experimental setups.

In Indonesian laboratories, TCI A5514 is widely used in biochemical, pharmacological, and food science research. It plays a key role in studies related to glycan structures, enzyme activity, and molecular interactions. Researchers in both academic and industrial settings rely on this compound for its ability to provide clear and reproducible results. Its application in labeling and identification processes supports the advancement of scientific knowledge and innovation in the region.

Advantages (TCI brochure)

Glyco-moiety and isothiocyano group react with an amino group to form a thiourea
Resulting thiourea is stable and reaches the detector without decomposition in reversed phase HPLC
Racemic amines form diastereomers that are separated efficiently by reversed phase HPLC

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 2
Reagents for Glyco Chemistry & Biology 5th Edition · p. 40

Related TCI products

Glycan Structure Analysis: TCI A5514 is used to label and analyze glycan structures, helping researchers understand their role in biological systems and disease mechanisms.
Drug Development: The compound aids in the identification of glycan modifications in drug molecules, supporting the development of more effective therapeutic agents.
Diagnostic Research: It is employed in diagnostic applications to track biomarkers and improve the accuracy of medical testing procedures.
Enzyme Activity Studies: TCI A5514 enables the study of enzyme interactions with glycan substrates, providing insights into metabolic pathways and cellular functions.
Food Science Investigations: The compound assists in analyzing carbohydrate structures in food products, contributing to nutritional and safety research.

From the TCI brochure

Amino acids (optical purity determination)
Propranolol
Trimetoquinol

Brand	TCI
CAS number	14152-97-7
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Glycoscience
Pack sizes	—
Physical form	—
Storage	Store in a cool, dry place away from light and moisture

Data from the TCI brochure

Panjang gelombang deteksi UV	254 nm (Kolom Kaseisorb LC ODS Super 4.6 mm I.D.x150 mm; 10 mM buffer fosfat/metanol = 45/55 (pH 3.0); 25 C; 1 mL/min)

Source: TCI brochure A1096 (2026-03-10)

TCI A5514 should be stored in a cool, dry environment, away from direct light and moisture to maintain its chemical integrity. It is recommended to use airtight containers made of glass or high-density polyethylene to prevent contamination and degradation. Due to its reactivity, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. The compound should be stored in a well-ventilated area to minimize exposure to vapors. While no specific storage temperature is provided, maintaining a stable room temperature is advised. Proper labeling and secure storage are essential to ensure safe handling and prevent accidental exposure in laboratory settings.

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