TCI

Bromophenol Blue (0.04% in Water) [for pH Determination] TCI B0117

Chemical Identity

PubChem: CID 8272·SID 87563315
Formula: C19H10Br4O5S
Molecular weight: 670.0 g/mol
Reference databases: ChEBI·ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-2,1lambda6-benzoxathiol-3-yl]phenol
SMILES: C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br
InChIKey: UDSAIICHUKSCKT-UHFFFAOYSA-N

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Volume 500mL

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Bromophenol Blue is a widely used pH indicator in laboratory settings, particularly for determining the acidity or alkalinity of solutions. It is commonly employed in analytical chemistry for its reliable color change response across a specific pH range, making it an essential tool for qualitative and quantitative pH analysis. This reagent is available in a 0.04% aqueous solution, which ensures ease of use without interfering with the measurement process. Its role in pH determination is critical in various scientific investigations, especially in environmental and food chemistry.

The chemical properties of Bromophenol Blue contribute to its effectiveness as a preferred choice in laboratory applications. It exhibits a stable color change from blue to yellow as the pH shifts from 4.0 to 6.0, providing clear and consistent results. Its chemical stability under controlled conditions allows for accurate and repeatable measurements. Additionally, its resistance to degradation and ability to maintain color integrity over time make it a reliable reagent for long-term use. These characteristics ensure that it remains a trusted component in analytical procedures.

In Indonesian laboratories, Bromophenol Blue is frequently used in quality control processes for water, food, and chemical samples. Its ease of handling and consistent performance make it a standard reagent in both academic and industrial settings. The 0.04% concentration ensures that it is neither too concentrated nor too dilute, allowing for precise and accurate pH measurements. Its reliability and simplicity make it a staple in the analytical chemistry toolkit of Indonesian research facilities.

TCI brochures (PDF)

Titration Reagents and TLC Stains 2026-05-07 · p. 2

Related TCI products

pH Titration: Bromophenol Blue is ideal for titration experiments due to its distinct color change in the pH range of 4.0 to 6.0, allowing for accurate endpoint detection in acid-base reactions.
Environmental Analysis: It is commonly used in water quality testing to assess the pH levels of natural and treated water samples, ensuring compliance with environmental standards.
Food Chemistry: This reagent is used in food testing to determine the pH of various food products, which is crucial for quality control and safety assessments.
Educational Laboratories: Bromophenol Blue is widely used in teaching labs to demonstrate pH indicators and their behavior in different solutions, supporting student learning.
Industrial Quality Control: It is employed in industrial settings to monitor pH levels in chemical processes, ensuring product consistency and safety.

Brand	TCI
CAS number	—
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > pH Measurement Reagents
Pack sizes	Available in standard laboratory quantities
Physical form	Aqueous solution
Storage	Store in a cool, dark place away from direct sunlight

Bromophenol Blue should be stored in a cool, dark place to maintain its chemical stability and color integrity. It is recommended to keep the reagent in a tightly sealed container to prevent contamination and evaporation. Avoid exposure to direct sunlight and high temperatures to ensure the solution remains effective for its intended use. In laboratory settings, it is important to handle the reagent with care, using appropriate personal protective equipment when necessary. Proper labeling of containers is essential to prevent mix-ups and ensure safe handling. Regular inspection of storage conditions helps maintain the quality and reliability of the reagent for accurate pH measurements.

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