5-Bromo-4-chloro-3-indolyl beta-D-Glucuronide Sodium Salt is a chemical compound widely used in biochemical and molecular biology research. It serves as a substrate for beta-glucuronidase, an enzyme involved in the hydrolysis of beta-glucuronides. This compound plays a crucial role in enzymatic reactions, enabling researchers to measure enzyme activity accurately. Its application is essential in various laboratory experiments, particularly those focusing on enzyme kinetics and metabolic pathways. The compound's specificity for beta-glucuronidase makes it a reliable tool for quantitative analysis in biochemical studies.
The compound is valued for its stability and reactivity, which ensure consistent results in experimental settings. Its sodium salt form provides good solubility in aqueous solutions, making it easy to handle and use in standard laboratory procedures. This solubility enhances its utility in a range of biochemical assays. Additionally, its chemical structure allows for specific interactions with beta-glucuronidase, ensuring accurate and reproducible data. These properties make it a preferred choice for researchers working in biochemistry and molecular biology.
In Indonesian laboratories, this compound is commonly used in molecular biology and biochemical research. It is a key reagent in experiments that require the measurement of enzyme activity, especially in studies related to metabolism and microbial ecology. Its reliability and performance in controlled environments make it an essential component in many research projects conducted in academic and industrial settings across Indonesia.
TCI brochures (PDF)
- Substrates for Biochemical Assays 2025-04-25 · p. 6
- Reagents for Biological Research 2025-03-11 · p. 20
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- Biochemical Assays - This compound is ideal for measuring beta-glucuronidase activity in enzyme kinetics studies due to its specific interaction with the enzyme.
- Molecular Biology Research - It is used in experiments that require precise quantification of enzymatic reactions, supporting studies in gene expression and protein function.
- Microbial Ecology Studies - The compound aids in analyzing microbial degradation processes, helping researchers understand how microorganisms break down complex compounds.
- Enzyme Inhibition Studies - Its use allows for the evaluation of enzyme inhibitors, providing insights into enzyme mechanism and function.
- Metabolic Pathway Analysis - It supports research into metabolic pathways by enabling the measurement of enzyme activity in various biological systems.
| Brand | TCI |
|---|---|
| CAS number | 129541-41-9 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Enzymes, Enzyme Inhibitors, Enzyme Substrates |
| Pack sizes | Available in various quantities as per standard laboratory supply |
| Physical form | Sodium salt powder |
| Storage | Store in a cool, dry place away from moisture and light |
This compound should be stored in a cool, dry environment to maintain its stability and prevent degradation. It is recommended to keep it in a sealed container to avoid exposure to moisture and light, which can affect its chemical integrity. When handling, researchers should use appropriate personal protective equipment such as gloves and safety goggles to ensure safety. The compound is generally non-hazardous but should be treated with care as per standard laboratory protocols. It is important to ensure that the storage area is well-ventilated and free from incompatible substances. Proper storage and handling will help maintain the compound's effectiveness and ensure safe usage in laboratory settings.
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