5-Bromo-4-chloro-3-indolyl beta-D-Galactopyranoside [for Biochemical Research] TCI B3201

Chemical Identity

Other names: X-Gal · HPLC(N)
CAS No.: 7240-90-6
PubChem: CID 44629973·SID 87560918
Formula: C14H15BrClNO6
Molecular weight: 408.63 g/mol
Purity: >98.0%(HPLC)(N)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (2S,3S,5R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
SMILES: C1=CC(=C(C2=C1NC=C2O[C@H]3[C@H](C([C@H](C(O3)CO)O)O)O)Cl)Br
InChIKey: OPIFSICVWOWJMJ-QRZKCFAKSA-N

Rp 1.378.650,00

~~Rp 979.650,00~~ Rp 1.378.650,00

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Berat 200mg 1g

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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X-Gal, is a vital chemical compound used extensively in biochemical research, particularly in genetics and molecular biology. It serves as a substrate for the enzyme β-galactosidase, enabling the detection and visualization of gene expression in organisms with the lac operon promoter. This compound plays a crucial role in various experimental techniques, including Southern blotting, Western blotting, and the creation of reporter plasmids for promoter activity analysis. Its unique chemical structure, combining an indole ring with a β-D-galactose sugar, contributes to its specificity and reactivity in enzymatic reactions.

X-Gal is preferred due to its high reactivity and specificity in enzymatic assays. Its chemical stability under controlled conditions, combined with its sensitivity to light and oxidation, makes it a reliable yet carefully handled reagent. The compound’s ability to produce a visible color change upon enzymatic cleavage makes it ideal for detecting gene expression in both prokaryotic and eukaryotic systems. These properties make it an essential tool for researchers aiming to study promoter activity and gene regulation with precision.

In Indonesian laboratories, X-Gal is routinely used in genetic and molecular biology research across universities, research centers, and clinical laboratories. It is a key reagent in experiments involving gene expression, reporter plasmid construction, and enzyme activity assays. Its widespread use highlights its importance in advancing biotechnological and biomedical research in the region.

TCI brochures (PDF)

Substrates for Biochemical Assays 2025-04-25 · p. 6
Reagents for Glyco Chemistry & Biology 5th Edition · p. 18
Reagents for Biological Research 2025-03-11 · p. 19

Related TCI products

Southern blot analysis utilizes X-Gal to detect specific DNA sequences through enzymatic cleavage, offering high sensitivity and specificity for genetic studies.
Western blot techniques incorporate X-Gal to visualize protein expression by detecting enzymatic activity, aiding in the study of gene regulation and protein function.
Reporter plasmid construction relies on X-Gal to monitor promoter activity, enabling researchers to assess gene regulation in various experimental conditions.
Enzymatic activity assays use X-Gal as a substrate to measure β-galactosidase activity, providing insights into enzyme kinetics and functional studies.
Gene expression studies benefit from X-Gal’s ability to produce a visible color change, allowing for the detection of gene expression in both prokaryotic and eukaryotic systems.

Brand	TCI
CAS number	7240-90-6
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Glycoscience
Pack sizes	Available in various quantities as per standard laboratory reagent packaging
Physical form	Solid powder
Storage	Store in a cool, dark place away from light and oxidizing agents

X-Gal should be stored in a cool, dark environment to prevent degradation due to light exposure and oxidation. It is recommended to use opaque containers to minimize light exposure and maintain chemical integrity. The compound is sensitive to environmental factors, so it should be kept in a dry, well-ventilated area away from heat sources. Proper labeling and secure storage are essential to prevent contamination and ensure safe handling. Laboratory personnel should wear appropriate personal protective equipment when handling X-Gal to avoid direct contact. Regular monitoring of storage conditions is advised to maintain the compound’s effectiveness for experimental use.

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