6-Chloro-3-indolyl β-D-galactopyranoside is a chemical compound widely used in biochemical research, particularly in the study of carbohydrate metabolism and enzymatic activity. This compound serves as a substrate for β-galactosidase, a key enzyme in enzymatic and genetic analysis. Its role in laboratories is critical for assessing enzyme activity, especially in genetic and biochemical research contexts. It is a fundamental reagent for researchers aiming to understand enzyme function and genetic expression mechanisms.
The compound is preferred due to its high purity and specificity in reacting with β-galactosidase. Its molecular structure is stable and soluble in organic solvents, making it easy to handle and incorporate into various experimental protocols. These properties ensure reliable and consistent results in laboratory settings. Its stability and solubility also contribute to its effectiveness in different experimental conditions.
In Indonesian laboratories, 6-Chloro-3-indolyl β-D-galactopyranoside is commonly used by researchers in universities and research institutions. It plays a vital role in studies involving genes and proteins, especially in biotechnology and molecular biology. Its relevance in scientific research makes it an essential tool for advancing knowledge in these fields.
- Enzymatic Activity Assays: This compound is ideal for measuring β-galactosidase activity, providing accurate and reproducible results in enzymatic studies.
- Genetic Expression Analysis: It is used in reporter gene assays to monitor gene expression, offering a reliable method for studying genetic regulation.
- Molecular Biology Research: Its role as a substrate supports experiments in molecular biology, particularly in cloning and gene expression studies.
- Biochemical Pathway Studies: It aids in understanding carbohydrate metabolism and enzyme kinetics, contributing to broader biochemical research.
- Biotechnology Applications: It is valuable in biotechnology for developing and testing new enzymatic processes and genetic tools.
| Brand | TCI |
|---|---|
| CAS number | 138182-21-5 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Chemical Biology > Glycoscience |
| Pack sizes | Available in various quantities as per supplier specifications |
| Physical form | Solid powder |
| Storage | Store in a cool, dry place away from light |
This compound should be stored in a cool, dry environment, away from direct light to maintain its stability and effectiveness. It is recommended to use airtight containers to prevent moisture absorption and contamination. Due to its chemical nature, it should be handled with appropriate personal protective equipment, such as gloves and safety goggles, to ensure laboratory safety. Proper ventilation should be maintained when working with this compound to minimize exposure. It is important to follow standard laboratory safety protocols to ensure safe handling and storage practices.
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