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TCI

CAS 3150-24-1

4-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-Galactosidase] TCI N0616

4-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-Galactosidase] TCI N0616

Chemical Identity

CAS No.
3150-24-1
PubChem
CID 65115·SID 87573933
Formula
C12H15NO8
Molecular weight
301.25 g/mol
Purity
>98.0%(HPLC)
Reference databases
ChEBI·ChemSpider·ECHA
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
SMILES
C1=CC(=CC=C1[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O
InChIKey
IFBHRQDFSNCLOZ-YBXAARCKSA-N
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4-Nitrophenyl beta-D-Galactopyranoside is a chemical compound used as a substrate for beta-galactosidase enzymes. It plays a crucial role in biochemical and molecular biology laboratories, particularly in enzyme activity assays. This compound is widely utilized to measure the activity of beta-galactosidase, an enzyme involved in the breakdown of beta-galactose. Its specificity makes it a reliable tool for enzyme kinetics and metabolic studies. Due to its high reactivity and stability, it is a preferred choice for researchers aiming for accurate and reproducible results.

The compound exhibits chemical stability and is easily soluble in organic solvents such as ethanol and acetone. These properties make it suitable for a variety of experimental conditions. Its high purity and controlled reactivity ensure consistent performance in laboratory settings. Additionally, its availability in multiple packaging sizes allows for flexibility in experimental scale, from small-scale research to larger studies. This versatility enhances its utility across different laboratory applications.

In Indonesian laboratories, 4-Nitrophenyl beta-D-Galactopyranoside is commonly used in biochemical research, especially in enzyme studies and carbohydrate metabolism. It is a staple in molecular biology and cellular physiology experiments. Many research institutions and universities rely on this compound for its reliability and precision. Its application is essential for understanding enzymatic processes and their biological significance.

  • Enzyme Activity Assays: This substrate is ideal for measuring beta-galactosidase activity, enabling researchers to assess enzyme kinetics and catalytic efficiency.
  • Carbohydrate Metabolism Studies: It supports investigations into the breakdown of beta-galactose, providing insights into metabolic pathways and enzyme function.
  • Molecular Biology Research: Its use in enzyme assays aids in studying gene expression and protein function in cellular processes.
  • Biochemical Analysis: It is a key component in biochemical experiments requiring precise and reproducible enzyme activity measurements.
  • Cellular Physiology Investigations: It helps in understanding how enzymes interact with cellular components and influence metabolic processes.

Brand TCI
CAS number 3150-24-1
Molecular formula —
Purity —
Category Chemistry > Chemical Biology > Glycoscience
Pack sizes Available in various sizes to suit different experimental needs
Physical form Solid powder
Storage Store in a cool, dry place away from light

This compound should be stored in a cool, dry environment, away from direct light to maintain its chemical stability. It is recommended to use airtight containers to prevent moisture absorption and contamination. Due to its reactivity, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. It is important to ensure that the compound is stored separately from incompatible substances to avoid any potential chemical interactions. In laboratory settings, proper labeling and storage protocols are essential to maintain safety and prevent accidental exposure. Always follow standard laboratory safety practices when handling and storing this material.

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