4-Nitrophenyl alpha-D-Galactopyranoside [Substrate for alpha-D-Galactosidase] TCI N0492

Chemical Identity

CAS No.: 7493-95-0
PubChem: CID 82000·SID 87573818
Formula: C12H15NO8
Molecular weight: 301.25 g/mol
Purity: >98.0%(HPLC)
Reference databases: ChEBI·ChemSpider·ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
SMILES: C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O
InChIKey: IFBHRQDFSNCLOZ-IIRVCBMXSA-N

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4-Nitrophenyl alpha-D-Galactopyranoside is a chemical compound widely used as a substrate for alpha-D-galactosidase in biochemical and molecular biology experiments. This compound plays a key role in enzyme activity assays, particularly in the analysis of carbohydrate metabolism and glycan structure studies. Its ability to produce a measurable color change during enzymatic reactions makes it an essential tool for quantitative enzyme activity measurement. In laboratory settings, it enables researchers to assess enzyme function through spectrophotometric analysis, offering a reliable and reproducible method for enzyme detection and characterization.

The compound is chemically stable under specific conditions and readily reacts with alpha-D-galactosidase, ensuring accurate and sensitive detection. Its molecular structure is highly specific, allowing it to interact exclusively with the target enzyme and minimizing interference from other enzymes. This specificity enhances the reliability of experimental results. Additionally, its availability in solid form and ease of dissolution make it a practical choice for laboratory use, supporting both small-scale and larger research applications.

In Indonesian laboratories, 4-Nitrophenyl alpha-D-Galactopyranoside is commonly used in biochemical, pharmacological, and molecular biology research. It is a preferred reagent for enzyme activity testing, supporting studies in carbohydrate metabolism, glycan analysis, and enzyme kinetics. Its utility extends to academic and research institutions, where it aids in the development of new methodologies and the validation of experimental protocols.

TCI brochures (PDF)

Reagents for Glyco Chemistry & Biology 5th Edition · p. 23
Reagents for Biological Research 2025-03-11 · p. 22

Related TCI products

Enzyme Activity Assays: This substrate is ideal for measuring alpha-D-galactosidase activity due to its specific reaction with the enzyme and colorimetric output.
Carbohydrate Metabolism Studies: It supports research on how carbohydrates are broken down and processed in biological systems.
Glycan Structure Analysis: Its use in glycoscience helps in understanding the structure and function of complex carbohydrates.
Spectrophotometric Analysis: The compound’s ability to produce a measurable color change makes it suitable for quantitative enzyme activity testing.
Enzyme Kinetics Research: It provides a reliable tool for studying the rate and efficiency of enzyme reactions in controlled environments.

Brand	TCI
CAS number	7493-95-0
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Glycoscience
Pack sizes	Available in various quantities as per supplier specifications
Physical form	Solid
Storage	Store in a cool, dry place away from light

This compound should be stored in a cool, dry environment, away from direct sunlight and sources of heat. It is recommended to keep it in a sealed container to prevent moisture absorption and maintain its chemical stability. When handling, ensure that appropriate laboratory safety measures are followed, including the use of gloves and safety goggles to protect against potential skin or eye contact. The compound is not flammable, but it should be kept away from incompatible substances. Regular monitoring of storage conditions is advised to ensure the integrity of the material for experimental use. Proper labeling and storage practices help maintain the quality and usability of the compound in laboratory applications.

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