2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase] TCI N0418

Chemical Identity

Other names: ONPG
CAS No.: 369-07-3
PubChem: CID 96647·SID 87573757
Formula: C12H15NO8
Molecular weight: 301.25 g/mol
Purity: >98.0%(HPLC)
Reference databases: ChEBI·ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
SMILES: C1=CC=C(C(=C1)[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O
InChIKey: KUWPCJHYPSUOFW-YBXAARCKSA-N

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2-Nitrophenyl beta-D-Galactopyranoside is a chemical compound used as a substrate for the enzyme beta-D-galactosidase. It plays a critical role in biochemical and enzymatic analysis within laboratory settings. This compound is widely utilized in research to measure the activity of beta-D-galactosidase, an essential enzyme in carbohydrate metabolism. Its ability to react with the enzyme produces a colored product, making it ideal for colorimetric assays. This makes it a fundamental tool in biochemical studies and enzyme kinetics research.

The compound’s molecular structure allows it to bind efficiently with beta-D-galactosidase, ensuring accurate and reliable results in enzymatic reactions. Its chemical properties and reactivity make it a preferred choice in various laboratory applications. Its stability and predictable behavior under controlled conditions contribute to its reliability in experimental setups. These characteristics ensure consistent performance, which is vital for scientific accuracy and reproducibility.

In Indonesian laboratories, 2-Nitrophenyl beta-D-Galactopyranoside is commonly used in biochemical research, particularly in enzyme activity analysis and metabolic studies. It is a key component in experiments requiring precise measurement of enzymatic activity. Many research institutions and universities rely on this compound for its effectiveness and consistency in experimental procedures. Its use supports a wide range of scientific investigations in the field of glycoscience and chemical biology.

TCI brochures (PDF)

Substrates for Biochemical Assays 2025-04-25 · p. 6
Reagents for Glyco Chemistry & Biology 5th Edition · p. 23
Reagents for Biological Research 2025-03-11 · p. 20

Related TCI products

Enzyme Activity Assays: This compound is ideal for measuring beta-D-galactosidase activity due to its ability to produce a detectable color change when reacted with the enzyme.
Metabolic Pathway Studies: It is used to investigate carbohydrate metabolism by assessing the activity of beta-D-galactosidase in various biological samples.
Biochemical Research: Its role as a substrate makes it essential for studies involving enzyme kinetics and substrate specificity in glycoscience.
Colorimetric Analysis: The colored product formed during the reaction allows for precise and quantitative measurement of enzymatic activity.
Educational Experiments: It is commonly used in academic settings to teach students about enzyme function and biochemical reactions.

Brand	TCI
CAS number	369-07-3
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Glycoscience
Pack sizes	Available in various quantities as per supplier specifications
Physical form	Solid powder
Storage	Store in a cool, dry place away from light

This compound should be stored in a cool, dry place, away from direct light to maintain its stability and effectiveness. It is recommended to use airtight containers to prevent moisture absorption, which could affect its chemical integrity. Proper labeling of storage containers is essential for safe handling and easy identification. Laboratory personnel should wear appropriate personal protective equipment, such as gloves and safety goggles, when handling this material. It should be kept out of reach of children and stored separately from incompatible substances. Regular monitoring of storage conditions ensures the compound remains suitable for use in experimental procedures.

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