Basic Orange 14 TCI B6599

Chemical Identity

Other names: Acridine Orange Hemi(zinc chloride) Salt · 3,6-Bis(dimethylamino)acridinium Chloride Hemi(zinc chloride salt)
CAS No.: 10127-02-3
PubChem: CID 159263
Formula: C17H20ClN3.1/2ZnCl2
Molecular weight: 369.96 g/mol
Purity: >85.0%(HPLC)
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: zinc;3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine;dichloride;hydrochloride
SMILES: CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl.[Cl-].[Cl-].[Zn+2]
InChIKey: VADJQOXWNSPOQA-UHFFFAOYSA-L
MDL: MDL00081043

Rp 1.696.800,00

~~Rp 1.186.500,00~~ Rp 1.696.800,00

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Basic Orange 14 from TCI is a cationic dye of the acridine class, known in the literature as a salt form of acridine orange. Biology, microbiology and biochemistry laboratories have used it for a long time as a fluorescent stain. The compound interacts with nucleic acids and with acidic compartments inside cells. This makes Basic Orange 14 important for staining techniques and for visualizing cells under a fluorescence microscope. It is also used as a basic dye for general purposes.

Researchers choose this dye mainly for its distinctive fluorescence. The emission colour of acridine orange changes with the type of nucleic acid it binds to and with its surrounding environment. This lets researchers tell certain cell structures apart in a single staining step. As a cationic dye, the compound is readily drawn to negatively charged structures within the cell. Its solid salt form is convenient for preparing stock solutions to suit the needs of each protocol. TCI supplies this material with documented quality, so it suits research work that needs consistency from batch to batch.

In Indonesia, Basic Orange 14 is relevant to microbiology, cell biology and biochemistry laboratories at universities, research institutes and industrial quality-control facilities. Teaching laboratories can use it to demonstrate fluorescence staining. Research groups can use it in routine cell visualization work with fluorescence microscopes. Sourcing an established reagent such as TCI Basic Orange 14 through AMI Scientific helps laboratories keep their materials consistent across long-running projects and repeated experiments.

Related TCI products

Fluorescence microscopy of cells: the dye's distinctive fluorescence lets researchers see cell structures clearly under a fluorescence microscope, which supports routine imaging and observation work.
Nucleic acid staining: the compound interacts with nucleic acids, and its emission colour differs with the type of nucleic acid bound, so different structures can be distinguished in one staining.
Visualizing acidic cell compartments: because the dye builds up in acidic compartments within cells, it helps researchers observe and compare these regions during cell biology studies.
Microbiology staining work: microbiology laboratories have long used this cationic dye to stain microorganisms, because it is readily drawn to negatively charged cell structures.
General basic dye applications: besides fluorescence work, Basic Orange 14 serves as a basic dye for general laboratory purposes, and its solid salt form is easy to make into stock solutions.

Brand	TCI (product code B6599)
CAS number	10127-02-3
Molecular formula	—
Purity	—
Category	TCI
Pack sizes	please check the available pack options on the product listing or ask AMI Scientific
Physical form	solid salt form of a cationic acridine dye
Storage	follow the storage conditions on the TCI label and Safety Data Sheet

Keep Basic Orange 14 in its original, tightly closed container in a cool, dry and well-ventilated place. Protect it from direct light and moisture, and follow the storage conditions on the TCI label and Safety Data Sheet. Prepare stock solutions in clean, labelled containers that suit the protocol, and protect them from light when they are not in use. Acridine dyes interact with nucleic acids, so handle this material with care. Wear gloves, a lab coat and eye protection, avoid creating dust, and work in a ventilated area. Wash your hands after handling it, and dispose of the material and any solutions according to your institution's chemical waste procedures.

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