Basic Orange 14 (TCI B6599, CAS 10127-02-3) is a cationic acridine dye, widely known in the laboratory as acridine orange. It is a fluorescent, metachromatic stain that binds nucleic acids and accumulates in acidic cellular compartments such as lysosomes. These properties make it a long-standing tool in fluorescence microscopy, cell biology, and microbiological preparations, as well as a model cationic dye in adsorption and photophysics studies. Supplied as an orange powder in a 25 g pack, it should be handled with gloves and eye protection and stored cool, dry, and protected from light.
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- Wang et al. (2009). Interaction of bovine serum albumin with Acridine Orange (C.I. Basic Orange 14) and its sonodynamic damage under ultrasonic irradiation. Dyes and Pigments doi:10.1016/j.dyepig.2008.07.013
- Hu et al. (2017). Fe3O4 Magnetic Nanoparticles Modified with Sodium Dodecyl Sulfate for Removal of Basic Orange 21 and Basic Orange 22 from Complex Food Samples with High-Performance Liquid Chromatographic Analysis. Food Analytical Methods doi:10.1007/s12161-017-0878-2
- Ikram et al. (2022). Biological Degradation of the Azo Dye Basic Orange 2 by Escherichia coli: A Sustainable and Ecofriendly Approach for the Treatment of Textile Wastewater. Water doi:10.3390/w14132063
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