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Description
TCI C2237 Chlorzoxazone is a chlorinated benzoxazolone compound supplied as a research reagent, and it has long been recognised in the pharmacological literature. In the laboratory, this compound is most often used not as a synthetic building block but as a chemical probe — a molecule whose behaviour in biological systems has been mapped well enough that it can be used to interrogate other systems. Its roles span drug metabolism investigations, microsomal enzyme studies, and microbiology experiments examining how small molecules influence bacterial cell behaviour and communication.
The properties that make this compound a preferred choice are its very well-defined metabolic profile and the availability of established analytical methods for detecting both the parent compound and its hydroxylated product. The benzoxazolone structure is sufficiently stable under ordinary storage conditions, and the material presents as a crystalline solid that is easy to weigh accurately. It dissolves in common organic solvents such as DMSO, so stock solutions are straightforward to prepare for cell-based experiments. The chlorinated aromatic ring also confers adequate lipophilicity for crossing cell membranes, an important consideration in microbiology work.
In Indonesian laboratories, the compound is typically encountered in university research groups and institutional research units working on drug metabolism, enzymology, and microbiology. It is used at analytical and cell-culture scale rather than in bulk preparative work, with weighed portions taken from the crystalline solid and diluted into DMSO stock solutions. Because the reagent is intended for research purposes, it is normally handled within a controlled laboratory workflow supported by documented analytical methods.
Laboratory Applications
Drug metabolism studies — the compound has a very well-defined metabolic profile, making it a dependable reference substrate when characterising metabolic conversion in experimental systems.
Microsomal enzyme studies — established analytical methods allow both the parent compound and its hydroxylated product to be detected, which supports quantitative work on microsomal enzyme activity.
Quorum sensing and bacterial communication research — its adequate lipophilicity supports membrane penetration, allowing investigation of how small molecules affect bacterial cell communication.
Chemical probe applications — because its behaviour in biological systems is already mapped, it can be applied to interrogate less well-characterised biological systems with confidence.
Cell-based assay development — its solubility in common organic solvents such as DMSO makes preparation of stock solutions for cell-based experimental protocols straightforward and reproducible.
Specifications
Brand: TCI
CAS Number: 95-25-0
Category: Life Science > Microbiology > Quorum Sensing Research
Pack sizes: available in standard research-scale packaging as listed for this catalogue item
Physical form: crystalline solid, readily weighed
Storage: stable under ordinary storage conditions; store in a closed container away from moisture
Handling and Storage
Store the material in its original tightly closed container under ordinary laboratory storage conditions, protected from moisture and direct light. The benzoxazolone structure is sufficiently stable for routine storage, but containers should be resealed promptly after each use to preserve the crystalline solid in a free-flowing, easily weighed state. Prepare DMSO stock solutions in clean, chemically compatible glass or polypropylene vessels and label them clearly with contents and preparation date. Handle the powder in a well-ventilated area or fume hood, wearing a laboratory coat, gloves, and eye protection, and avoid generating dust during weighing. This is a research reagent and should be used only within a controlled laboratory workflow by trained personnel, following the supplied safety data sheet and institutional waste disposal procedures.
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