• Disodium beta-Glycerophosphate Pentahydrate [for Biochemical Research] (CAS 13408-09-8) - TCI D3964 - AMI Scientific
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CAS 13408-09-8

Disodium beta-Glycerophosphate Pentahydrate [for Biochemical Research] TCI D3964

Disodium beta-Glycerophosphate Pentahydrate [for Biochemical Research] TCI D3964
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Disodium beta-Glycerophosphate Pentahydrate [for Biochemical Research] is the sodium salt of beta-glycerophosphate supplied in its pentahydrate form. The compound is a simple organic phosphate ester that is widely used as a source of organic phosphate in biological systems, as a model substrate for phosphatase enzymes, and as a buffering component in a range of working solution formulations. In biochemistry and cell biology laboratories, this material serves as one of the routine reagents supporting enzymology experiments, cell culture work, and protein analysis that requires careful control of phosphatase activity.

The characteristics that make this reagent a preferred choice begin with its good solubility in water, a direct consequence of the disodium salt form, so that stock solutions can be prepared easily without the need for organic solvents. As an organic phosphate analogue, the compound acts as a serine/threonine phosphatase inhibitor and is commonly added to lysis buffers to preserve the phosphorylation state of proteins so that it is not lost during sample preparation. The pentahydrate form gives a stable solid that is straightforward to weigh out, while the grade intended for biochemical research makes it suitable for both enzyme systems and cell-based work.

In Indonesian laboratories, this reagent typically appears in university research groups, biochemistry and molecular biology teaching laboratories, hospital and clinical research units, and industrial R&D laboratories working with proteins and enzymes. It is generally stocked as a routine bench chemical for preparing lysis buffers and enzyme assay solutions, where consistent phosphatase control is needed across repeated experiments. Its water solubility and stable solid form make it practical for laboratories preparing their own working solutions in-house.

  • Phosphatase inhibition in lysis buffers: added during cell and tissue lysis so that serine/threonine phosphatase activity is suppressed and protein phosphorylation status is preserved throughout sample preparation for downstream analysis.
  • Model substrate for phosphatase enzyme assays: as a simple organic phosphate ester, it provides a well-defined substrate for characterising phosphatase activity in enzymology experiments and comparative enzyme studies.
  • Organic phosphate source in biological systems: supplies phosphate in an organic ester form for experimental systems that require an available phosphate source rather than free inorganic phosphate.
  • Buffering component in working solution formulations: incorporated into a range of laboratory buffer formulations where its salt form contributes to the composition of prepared working solutions.
  • Protein analysis and cell culture support: used as a routine reagent in cell biology and protein workflows where phosphatase activity must be carefully controlled to obtain reproducible and interpretable results.

Brand TCI
CAS number 13408-09-8
Molecular formula —
Purity —
Category Life Science > Biochemicals and Reagents > Enzymes, Enzyme Inhibitors, Enzyme Substrates
Pack sizes please refer to the manufacturer's packaging and label information
Physical form —
Storage —
  • Product code: D3964
  • Grade: for biochemical research; supplied as a stable solid in the pentahydrate form

Store the container tightly closed in a cool, dry place away from moisture, following the storage conditions stated on the manufacturer's label. As a hydrated salt, the material should be protected from humidity so that its solid form and ease of weighing are maintained. Keep it in its original container or in a clean, well-sealed chemical container that is clearly labelled. Handle the reagent in a well-ventilated laboratory area using standard personal protective equipment, including a laboratory coat, gloves, and safety glasses. Avoid generating dust while weighing, use clean and dry spatulas to prevent contamination of the stock, and prepare aqueous solutions with laboratory-grade water. Consult the manufacturer's Safety Data Sheet before use and dispose of any waste according to applicable laboratory waste procedures.

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