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Description
Beta-Nicotinamide Adenine Dinucleotide Disodium Salt (NADH) is a critical biochemical compound widely used in laboratory research. As a reduced form of NAD, it plays a vital role in enzymatic reactions, particularly in redox processes essential for cellular metabolism. NADH functions as a coenzyme and substrate in various biochemical pathways, supporting the activity of enzymes such as alcohol dehydrogenase and lactate dehydrogenase. Its presence is crucial for energy metabolism and metabolic regulation in biological systems. This compound is indispensable for researchers studying metabolic processes and enzymatic mechanisms.
NADH is valued for its chemical stability under controlled conditions and its high solubility in water, which facilitates its use in a wide range of biochemical applications. The disodium salt form enhances its bioavailability, making it easier to incorporate into experimental setups. Its sensitivity to oxidation and light necessitates careful handling and storage to preserve its functional integrity. These properties make NADH a preferred choice for laboratories requiring reliable and consistent performance in biochemical assays.
In Indonesian laboratories, NADH is extensively used in biomedical, pharmacological, and biotechnological research. It supports studies on cellular metabolism, enzyme activity, and biochemical reaction mechanisms. Researchers in universities and research institutions rely on NADH for experiments that require precise control over redox reactions and metabolic pathways. Its versatility and reliability make it a fundamental component in many experimental protocols.
Laboratory Applications
Biochemical Research: NADH is essential for studying redox reactions and metabolic pathways, providing a stable and reliable substrate for enzyme assays.
Enzyme Activity Studies: It serves as a coenzyme in experiments involving alcohol dehydrogenase and lactate dehydrogenase, enabling accurate measurement of enzymatic activity.
Metabolic Pathway Analysis: NADH supports investigations into cellular energy metabolism and the role of NAD in metabolic regulation.
Biomedical Research: It is used in studies related to cellular respiration, oxidative stress, and the effects of metabolic disorders on biological systems.
Pharmacological Studies: NADH aids in evaluating drug interactions and the impact of compounds on metabolic processes and enzyme function.
Specifications
Brand: TCI
CAS number: 606-68-8
Category: Life Science > Biochemicals and Reagents > Enzymes, Enzyme Inhibitors, Enzyme Substrates
Pack sizes: Available in various quantities as per standard laboratory requirements
Physical form: Sodium salt of NADH, typically in powder form
Storage note: Should be stored in a cool, dark place to prevent oxidation and maintain stability
Handling and Storage
NADH should be stored in a cool, dark environment to prevent oxidation and maintain its chemical integrity. It is recommended to keep the compound in airtight containers to minimize exposure to moisture and light. Due to its sensitivity, it is advisable to use it promptly after preparation to ensure optimal performance in experiments. Proper labeling and storage conditions are essential to prevent degradation and contamination. Laboratories should handle NADH with care, using appropriate safety measures when handling and preparing solutions. Maintaining a controlled storage environment ensures the reliability and consistency of experimental results.
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