17alpha-Hydroxyprogesterone TCI H1250

TCI H1250, with CAS number 68-96-2, is 17alpha-Hydroxyprogesterone, a naturally occurring steroid hormone belonging to the progestogen class and classified as a life science biochemical. The compound is an important intermediate metabolite in the steroid biosynthesis pathway, sitting at the junction between cortisol formation and sex hormone production. In research and testing laboratories it serves as an analytical reference material, as a calibration standard for both immunoassay and chromatographic methods, and as a test substance in endocrinology and steroid metabolism studies at the cellular and tissue level.

What makes this compound important is its role as a well-established biochemical marker. Levels of 17alpha-hydroxyprogesterone in biological samples are widely used as an indicator in research into disorders of adrenal steroid biosynthesis, which makes the availability of reference material with a clearly defined identity an absolute requirement for the development and validation of analytical methods. Its characteristic four-ring steroid structure gives the compound lipophilic properties, limited solubility in water, and good solubility in organic solvents such as ethanol, methanol, and dimethyl sulfoxide. These characteristics determine how working solutions are prepared.

In Indonesian laboratories, 17alpha-Hydroxyprogesterone is typically handled in university research laboratories, clinical chemistry and endocrinology research units, and analytical method development groups. It is used wherever a defined reference substance is needed to build calibration curves, verify instrument response, or support studies of steroid metabolism. Because the material is supplied as a characterised reference substance from TCI, it fits routine workflows in which method validation and analytical traceability are part of the standard laboratory practice.

• Analytical reference material: the compound provides a clearly identified substance against which sample responses can be compared during identification and confirmation work in steroid analysis.
• Calibration standard for immunoassay methods: it is used to construct and verify calibration curves so that assay response can be related reliably to 17alpha-hydroxyprogesterone content in the samples tested.
• Calibration standard for chromatographic methods: the defined identity of the material supports retention time assignment and quantitative calibration in chromatographic separations of steroid compounds.
• Endocrinology research: as an intermediate metabolite positioned between cortisol formation and sex hormone production, it serves as a test substance in studies of hormone pathways at cellular and tissue level.
• Steroid metabolism studies: researchers use the compound to investigate biosynthetic conversion steps, making it suitable for cell-based and tissue-based experimental work on adrenal steroid biosynthesis.

• Chemical name: 17alpha-Hydroxyprogesterone
• Compound class: naturally occurring steroid hormone of the progestogen group

Store the material in its original tightly closed container, protected from light, moisture, and elevated temperature, and follow the storage conditions stated on the manufacturer's label and safety data sheet. Amber glass containers are suitable for both the solid material and prepared solutions. Because the compound is lipophilic and only sparingly soluble in water, working solutions are normally prepared in organic solvents such as ethanol, methanol, or dimethyl sulfoxide. Handle the material as a biologically active steroid hormone: weigh it in a well-ventilated area or fume hood, wear gloves, a laboratory coat, and eye protection, avoid the generation and inhalation of dust, and allow the container to reach room temperature before opening to limit condensation. Dispose of residues and contaminated consumables in accordance with applicable laboratory waste procedures.

—