1-Methoxy-5-methylphenazinium Methyl Sulfate [for Biochemical Research] TCI M1442

Chemical Identity

CAS No.: 65162-13-2
PubChem: CID 127832·SID 87559448
Formula: C15H16N2O5S
Molecular weight: 336.4 g/mol
Purity: >95.0%(HPLC)
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 1-methoxy-5-methylphenazin-5-ium;methyl sulfate
SMILES: C[N+]1=C2C=CC=C(C2=NC3=CC=CC=C31)OC.COS(=O)(=O)[O-]
InChIKey: MASUWVVNWALEEM-UHFFFAOYSA-M

Rp 3.048.150,00

Sale Sold out

Shipping calculated at checkout.

Berat 200mg

Pembayaran hanya melalui request quotation dan dilakukan setelah tim kami menghubungi Anda. Mohon cantumkan nomor WhatsApp aktif untuk konfirmasi pesanan & pembuatan invoice. Estimasi pengiriman 2-4 minggu setelah pembayaran.

Dokumen Keamanan & Mutu

↓ Unduh SDS / MSDS (PDF resmi TCI)

CoA (Certificate of Analysis)

Spesifik per batch produksi — diterbitkan dan dikirim bersama barang sesuai nomor lot yang Anda terima.

Contoh CoA segera tersedia.

💬 Minta CoA via WhatsApp

View full details

TCI M1442 1-Methoxy-5-methylphenazinium Methyl Sulfate, referred to in much of the published literature as 1-Methoxy PMS, is a phenazinium compound that functions as an electron transfer mediator in cell-based biological assays. Its role in the laboratory is to bridge the movement of electrons from the dehydrogenase systems inside living cells to the tetrazolium salt reagent used as a colorimetric indicator. Without a mediator of this kind, many water-soluble tetrazolium reagents cannot be reduced efficiently, so the absorbance signal that is read becomes weak and less reliable as a basis for concluding the degree of cell proliferation or cell viability.

The principal characteristics that make this compound a preferred choice are its efficiency as an electron carrier and its better stability compared with earlier-generation phenazine methosulfate reagents, particularly with respect to decomposition caused by light. This property matters because cell viability assays frequently require incubation of several hours inside an incubator, so a mediator that degrades quickly will reduce the reproducibility of results between wells as well as between plates. The compound is soluble in water, which makes it straightforward to prepare as a stock solution and to combine with the tetrazolium reagent used in the assay.

In Indonesian laboratories, this material is used in the same working context as elsewhere: cell biology laboratories, university research groups, and biomedical and pharmacological testing facilities that run proliferation and viability measurements on cultured cells. It is typically handled alongside water-soluble tetrazolium reagents within a routine microplate workflow, prepared as an aqueous stock solution and added to the assay system at the point where the colorimetric reading is to be developed.

Related TCI products

Cell proliferation assays: it supplies the electron transfer step that allows tetrazolium reagents to be reduced efficiently, producing a colorimetric signal proportional to metabolically active cells.
Cell viability assays: as a mediator it ensures that reducing equivalents generated by dehydrogenase systems in living cells reach the indicator reagent, so weak or ambiguous absorbance readings are avoided.
Cytotoxicity screening of test compounds: reliable mediation of the colour-forming reaction supports the comparison of treated and untreated wells on the same plate under identical incubation conditions.
Microplate-format colorimetric work requiring multi-hour incubation: its improved stability against light-induced decomposition helps maintain reproducibility between wells and between plates during long readings.
General biochemical research on dehydrogenase-linked reactions: as an efficient electron carrier it is used where electrons must be shuttled from an enzymatic system to an external acceptor reagent.

Brand	TCI (Tokyo Chemical Industry)
CAS number	65162-13-2
Molecular formula	—
Purity	—
Category	Life Science > Cell Biology > Cell Proliferation Assay and Cell Viability Assay
Pack sizes	available in the catalogue pack sizes offered by TCI for this item; storage should follow the manufacturer's stated conditions on the product label
Physical form	—
Storage	—

Grade / intended use: for biochemical research
Solubility: soluble in water; readily prepared as an aqueous stock solution

Store the compound according to the storage conditions stated by the manufacturer on the product label and in its accompanying documentation. Because this phenazinium mediator is subject to decomposition on exposure to light, containers should be protected from light and kept tightly closed, and amber or otherwise light-shielded vessels are appropriate for both the solid material and any aqueous stock solution prepared from it. Handle the material in a clean laboratory area using standard personal protective equipment, including gloves, laboratory coat, and eye protection, and avoid the generation of dust when weighing. Prepared stock solutions should be clearly labelled with the contents and the preparation date, kept protected from light, and handled with attention to the cleanliness required for cell culture work. Dispose of residues and contaminated consumables in accordance with the applicable laboratory chemical waste procedures.

—