Phenazine Methyl Sulfate (PMS) is a chemical compound widely used in biochemical and cell biology research. It serves as a key reagent in laboratory experiments, particularly in assays that measure cell proliferation and viability. PMS is commonly employed in MTT and WST-1 assays, where it acts as an oxidizing agent to detect metabolic activity in living cells. Its ability to bind with tetrahydrofuran (THF) and change color to blue makes it ideal for spectrophotometric analysis, enabling precise quantification of cell viability.
PMS is valued for its chemical stability under controlled conditions and its strong oxidizing properties. These characteristics allow for rapid and efficient chemical reactions, making it a preferred choice in biochemical analysis. Its sensitivity to light and moisture requires careful handling, but when stored properly, it maintains its effectiveness. The compound’s reliability and consistency in performance make it a trusted reagent in various research applications.
In Indonesian laboratories, PMS is frequently used in biomedical, pharmacological, and biotechnological research. It plays a crucial role in studies involving cell growth, drug efficacy testing, and metabolic activity assessments. Its widespread use underscores its importance in supporting scientific investigations that require accurate and reproducible results.
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- Cell Proliferation Assays: PMS is used to assess cell growth and division by detecting metabolic activity through colorimetric changes.
- Cell Viability Testing: It enables the measurement of live cell populations in assays such as MTT and WST-1, providing reliable data on cell health.
- Biochemical Reaction Analysis: Its strong oxidizing properties make it suitable for rapid and efficient chemical reactions in metabolic studies.
- Drug Screening Protocols: PMS supports the evaluation of drug effects on cell metabolism and viability in pharmaceutical research.
- Biotechnology Research: It is integral to experiments involving cell-based assays and metabolic pathway analysis in biotech applications.
| Brand | TCI |
|---|---|
| CAS number | 299-11-6 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Cell Biology > Cell Proliferation Assay and Cell Viability Assay |
| Pack sizes | Available in various quantities as per standard laboratory reagent packaging |
| Physical form | Solid powder |
| Storage | Store in a cool, dark place away from moisture and light |
Phenazine Methyl Sulfate should be stored in a cool, dark environment to prevent degradation due to light and moisture exposure. It is recommended to use airtight containers to maintain its chemical stability. Proper labeling and secure storage are essential to avoid contamination and ensure safety in the laboratory. Handling should be done with appropriate personal protective equipment, such as gloves and safety goggles, to minimize direct contact. Regular inspection of storage conditions is advised to maintain the integrity of the compound. Always follow standard laboratory safety protocols when working with reactive chemicals.
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