2'-C-Methyluridine TCI M3153

TCI M3153 2'-C-Methyluridine is a pyrimidine nucleoside analogue that carries an additional methyl group at the 2' position of the ribose sugar. This small modification to the uridine framework has a large effect on the biological behaviour of the molecule, making the compound an important starting material and test substance in antiviral research and in oligonucleotide chemistry. In the laboratory it serves as a precursor for the synthesis of modified nucleotides, as an analytical reference material, and as a model molecule for studying how changes to the sugar influence recognition by viral polymerases and by cellular kinases.

The characteristic that leads researchers to select this analogue is the methyl group on the 2' carbon, which is quaternary in nature and locks the conformation of the sugar ring. As a result, nucleotides derived from it can still be recognised by viral polymerases, yet they terminate elongation of the RNA chain once incorporated. This 2'-C-methyl motif is the design basis for many inhibitors of viral RNA polymerases. In addition, the still-free 2' and 3' hydroxyl groups together with the primary 5' hydroxyl provide three distinct points of functionalisation, allowing chemists to install protecting groups selectively in order to build phosphoramidites.

Within Indonesian laboratories, this reagent is typically found in university research groups, biochemistry and medicinal chemistry laboratories, and institutional research centres working on antiviral compounds and nucleic acid chemistry. It is normally handled at the bench scale for synthetic work, used as a comparison standard during analytical method development, or applied in enzymatic assays examining polymerase and kinase behaviour. Because it is a research chemical, it is intended for laboratory and research use rather than clinical or diagnostic application.

• Antiviral research: the 2'-C-methyl motif underpins the design of viral RNA polymerase inhibitors, making this analogue a direct reference point for structure-activity studies on chain-terminating nucleoside candidates.
• Modified nucleotide synthesis: the compound functions as a precursor from which mono-, di-, and triphosphate derivatives bearing the 2'-C-methyl sugar modification can be prepared for downstream biochemical testing.
• Oligonucleotide chemistry: the free 2' and 3' hydroxyls plus the primary 5' hydroxyl give three distinguishable functionalisation points, so chemists can apply protecting groups selectively and construct phosphoramidite building blocks.
• Polymerase and kinase recognition studies: because the quaternary 2' methyl group locks the sugar ring conformation, the molecule serves as a model for probing how sugar geometry affects enzymatic recognition and processing.
• Analytical reference material: the compound is used as a comparison standard in chromatographic and spectroscopic method development where a defined modified nucleoside is required for peak identification.

• Product code: M3153

Store the container tightly closed in a cool, dry place away from direct sunlight, moisture, and incompatible materials, following the storage conditions printed on the manufacturer's label and safety data sheet. Keep the material in its original supplier container, or transfer it to a clean, chemically compatible, well-sealed vial that is clearly labelled with the product name, CAS number, and date opened. Handle the compound in a well-ventilated area or fume hood using standard personal protective equipment, including a laboratory coat, safety glasses, and suitable gloves. Avoid generating dust when weighing the solid, and allow sealed containers to reach room temperature before opening if they have been kept refrigerated, so that condensation does not form on the material. Review the safety data sheet before first use and dispose of residues and contaminated consumables in accordance with applicable laboratory waste procedures.

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