TCI's n-Octane [for Spectrophotometry] (CAS 111-65-9) is a saturated, straight-chain aliphatic hydrocarbon with eight carbon atoms, supplied in a grade made specifically for spectrophotometric work. As a nonpolar solvent, n-octane dissolves hydrophobic organic compounds so they can be measured on a spectrophotometer, mainly in the ultraviolet and visible regions. The spectrophotometric grade is designed to keep background absorbance low, so the recorded signal comes from the analyte rather than from impurities in the solvent.
The main reason to choose spectrophotometric-grade n-octane is that it has no chromophore groups. As a saturated alkane, it absorbs little light across most of the UV-Vis range commonly used in laboratories, which makes it suitable as both a solvent and a blank. Because it is nonpolar, it interacts only weakly with dissolved compounds. The resulting spectra therefore resemble gas-phase spectra, with clearer vibrational structure. n-Octane also boils at a higher temperature than hexane, so it evaporates more slowly during measurement and keeps sample concentrations more stable.
In Indonesia, this solvent is used by analytical chemistry laboratories, university research groups, and industrial quality control units that run UV-Vis spectrophotometry on nonpolar organic compounds. Typical users are teams that characterise hydrophobic substances, prepare calibration standards in a nonpolar medium, or compare spectral behaviour across solvents of different polarity. These laboratories want a solvent that gives consistent readings from one batch to the next, and AMI Scientific supplies this TCI grade so they can obtain it within the country.
- UV-Vis sample preparation: n-octane dissolves hydrophobic organic compounds without adding chromophore absorption, so the measured spectrum reflects the analyte rather than the solvent.
- Reference blank measurements: low background absorbance in the spectrophotometric grade makes it a dependable blank for baseline correction when samples are dissolved in the same solvent.
- Studies of fine spectral structure: its nonpolar nature keeps solvent–solute interactions to a minimum, which helps preserve vibrational fine structure similar to gas-phase spectra.
- Solvent polarity comparisons: as a nonpolar, non-absorbing reference medium, n-octane is useful when researchers compare how solvent environment shifts or broadens absorption bands.
- Extended or repeated measurements: n-octane evaporates more slowly than hexane, which helps keep concentrations stable during longer runs or multiple scans of the same cuvette.
| Brand | TCI (product code O0118) |
|---|---|
| CAS number | 111-65-9 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Building Blocks > Non-Heterocyclic Building Blocks |
| Pack sizes | Available pack sizes follow the TCI catalogue; please confirm with AMI Scientific |
| Physical form | Liquid; keep tightly closed in a cool, well-ventilated place away from ignition sources |
| Storage | — |
- Grade: For Spectrophotometry
Store n-Octane [for Spectrophotometry] in its original, tightly sealed container in a cool, dry, well-ventilated area, away from heat, sparks, open flames, and strong oxidising agents. Like other volatile hydrocarbons, it is flammable, so keep it in a flammables cabinet and ground equipment during transfers where appropriate. Use glass or other compatible containers, and do not use plastics that may leach contaminants, because contamination would raise the solvent's background absorbance. Handle it in a fume hood while wearing chemical-resistant gloves, safety glasses, and a laboratory coat. Keep containers closed when not in use to limit evaporation and moisture uptake. Always consult the supplier's Safety Data Sheet for full hazard and disposal information.
—
![111-65-9 n-Octane [for Spectrophotometry] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510O0118.jpg?v=1767131405&width=1445)