Pyrogallol Red [for Protein Research] TCI P1976

Chemical Identity

CAS No.: 32638-88-3
PubChem: CID 360404·SID 253659869
Formula: C19H12O8S
Molecular weight: 400.4 g/mol
Purity: >95.0%(HPLC)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 2-(3,4,5-trihydroxy-6-oxoxanthen-9-yl)benzenesulfonic acid
SMILES: C1=CC=C(C(=C1)C2=C3C=CC(=O)C(=C3OC4=C2C=CC(=C4O)O)O)S(=O)(=O)O
InChIKey: JTZKNOUMMWIARI-UHFFFAOYSA-N

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TCI P1976 Pyrogallol Red [for Protein Research] is a sulfonephthalein dye made specifically for protein research. In biochemistry and clinical laboratories, pyrogallol red is best known as the main component of the pyrogallol red–molybdate method for determining total protein. This method is especially useful for samples with low protein levels, such as urine and cerebrospinal fluid. Analytical chemistry laboratories can also use the product as a complexometric indicator for metal ion analysis, which makes it a useful reagent across several disciplines.

The pyrogallol red–molybdate method works on a simple principle. Under acidic conditions, pyrogallol red forms a complex with molybdate ions. That complex then binds to the amino groups of proteins, which shifts the colour in a way that can be measured photometrically. Laboratories value the method because it is sensitive, fast, and easy to automate on chemistry analysers. The "for Protein Research" designation means the material's quality is prepared for this application. As a result, it gives a more consistent and reliable colour response, which matters when results are compared across runs, operators, or sample batches.

In Indonesia, urinary protein testing is an important parameter in research on kidney disease, preeclampsia, and diabetes. Biomedical research laboratories, clinical laboratories, and university biochemistry departments can use TCI P1976 Pyrogallol Red to prepare their own total protein reagents, to check method performance, or to teach the principles of dye-binding protein assays. Analytical chemistry laboratories can also use it in complexometric work, so one reagent can support both protein research and metal ion analysis.

TCI brochures (PDF)

Protein Determination Reagents 2026-03-31 · p. 5
Antibodies and Related Reagents 2026-07-29 · p. 27

Related TCI products

Total protein determination by the pyrogallol red–molybdate method: the dye forms a molybdate complex under acidic conditions that binds protein amino groups, which produces a colour shift that can be measured photometrically.
Low-concentration protein measurement in urine: the method's sensitivity makes it well suited to samples with low protein content, which is important in research on kidney disease, preeclampsia, and diabetes.
Protein analysis in cerebrospinal fluid: this sample type usually contains little protein, so a sensitive and consistent dye-binding reagent like this protein research grade helps produce dependable photometric readings.
Automated protein assays on chemistry analysers: the pyrogallol red–molybdate reaction is fast and easy to automate, so it suits laboratories that run large numbers of samples on analyser systems.
Complexometric indicator for metal ion analysis: as a sulfonephthalein dye, pyrogallol red can serve as an indicator in complexometric titrations of metal ions in analytical chemistry laboratories.

Brand	TCI (product code P1976)
CAS number	32638-88-3
Molecular formula	—
Purity	—
Category	Life Science > Molecular Biology > Proteomics
Pack sizes	available in TCI standard pack sizes; please confirm current options with AMI Scientific
Physical form	solid dye reagent; follow the storage conditions on the TCI label and Safety Data Sheet
Storage	—

Grade: for Protein Research

Keep TCI P1976 Pyrogallol Red in its original, tightly closed container. Store it in a cool, dry, well-ventilated place away from direct light, moisture, and incompatible substances, and always follow the specific conditions on the TCI label and Safety Data Sheet. Reseal the container promptly after each use so the dye does not absorb moisture or become contaminated, because either can change the colour response in protein assays. Prepare working reagent solutions in clean glassware or suitable chemical-resistant containers, and label them with the contents and preparation date. When handling the powder, wear standard laboratory personal protective equipment, including gloves, safety glasses, and a lab coat. Avoid breathing in dust and avoid contact with skin and eyes. Handle the material in a well-ventilated area or fume hood, and dispose of waste according to local regulations and your institution's procedures.

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