Bicinchoninic Acid Disodium Salt [for Protein Research] TCI B5838

Chemical Identity

Other names: Disodium 2,2'-Biquinoline-4,4'-dicarboxylate · 2,2'-Biquinoline-4,4'-dicarboxylic Acid Disodium Salt
CAS No.: 979-88-4
PubChem: CID 164763·SID 468590734
Formula: C20H10N2Na2O4
Molecular weight: 388.29 g/mol
Purity: >98.0%(HPLC)
Storage: 0-10°C
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: disodium;2-(4-carboxylatoquinolin-2-yl)quinoline-4-carboxylate
SMILES: C1=CC=C2C(=C1)C(=CC(=N2)C3=NC4=CC=CC=C4C(=C3)C(=O)[O-])C(=O)[O-].[Na+].[Na+]
InChIKey: AUPXFICLXPLHBB-UHFFFAOYSA-L
MDL: MDL00037500

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Bicinchoninic Acid Disodium Salt is a core reagent for the BCA protein assay, a widely used colorimetric method for total protein quantification in biological samples. It forms a purple-colored complex with Cu⁺ ions generated through the reduction of Cu²⁺ by peptide bonds under alkaline conditions, enabling accurate spectrophotometric measurement. This reagent is ideal for protein concentration determination prior to downstream applications such as electrophoresis, Western blotting, and enzymatic studies.