• 254973-02-9 AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling] - AMI Scientific
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TCI

CAS 254973-02-9

AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling] TCI A5576

AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling] TCI A5576

Chemical Identity

Other names
AABD-SH
CAS No.
254973-02-9
PubChem
CID 135888867·SID 87563193
Formula
C8H7N3O2S
Molecular weight
209.23 g/mol
Purity
>95.0%(HPLC)
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
N-(4-sulfanyl-2,1,3-benzoxadiazol-7-yl)acetamide
SMILES
CC(=O)NC1=CC=C(C2=NON=C12)S
InChIKey
FNYDOGDWHMLWTG-UHFFFAOYSA-N
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AABD-SH (4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) is a chemical compound widely used in analytical chemistry for derivatization processes. It plays a crucial role in enhancing the detection and identification of compounds through specific chemical reactions. This reagent is commonly employed in laboratory settings, particularly in High-Performance Liquid Chromatography (HPLC) for molecular labeling. Its ability to react with various functional groups makes it an essential tool for improving the sensitivity and accuracy of analytical procedures.

One of the key properties that make AABD-SH a preferred choice is its chemical stability and reactivity toward a wide range of organic compounds. Its molecular structure allows it to interact effectively with functional groups such as amines and hydroxyls, making it versatile for different derivatization applications. Additionally, its selective bonding capability enhances the specificity of analytical results. The compound is also resistant to common laboratory conditions, including moderate pH levels, which ensures consistent performance in various experimental setups.

In Indonesian laboratories, AABD-SH is extensively used in chemical, pharmaceutical, and environmental research. It is particularly valuable in educational institutions and research facilities where accurate and reliable analytical methods are required. Its role in improving chromatographic signal clarity supports precise quantification and qualitative analysis, making it an indispensable reagent in analytical chemistry workflows.

Advantages (TCI brochure)

  • Itself fluoresces very little, while thioester derivatives fluoresce highly
  • Thioester derivative stays intact under reversed phase HPLC
  • Detection limit 10-20 fmol (S/N = 3)

TCI brochures (PDF)

Related TCI products

  • High-Performance Liquid Chromatography (HPLC) utilizes AABD-SH for molecular labeling to enhance detection and identification of analytes through specific chemical reactions.
  • Pharmaceutical research benefits from AABD-SH due to its ability to react with functional groups, aiding in the analysis of complex drug compounds and metabolites.
  • Environmental analysis employs AABD-SH to improve the sensitivity of detecting trace organic pollutants in water and soil samples.
  • Chemical synthesis laboratories use AABD-SH for derivatization to improve the detectability of compounds during analytical procedures.
  • Academic research institutions rely on AABD-SH for its stability and reactivity, supporting accurate and reproducible analytical results in various studies.

From the TCI brochure

  • Labeling of carboxylic acids (fatty acids) by thioester formation

Brand TCI
CAS number 254973-02-9
Molecular formula —
Purity —
Category Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes Available in standard laboratory quantities as per supplier specifications
Physical form Solid or powder, depending on formulation
Storage Store in a cool, dry place away from direct sunlight and moisture

Data from the TCI brochure

Excitation wavelength368 nm (Fluorescence detection)
Emission wavelength524 nm (Fluorescence detection)
Detection limit10-20 fmol (S/N = 3)
ColumnKaseisorb LC ODS 2000, 4.6 mm I.D. x 150 mm (Chromatogram of fatty acids)
Mobile phase / flow rateCH3OH; 1 mL/min (Temperature ambient)

Source: TCI brochure A1096 (2026-03-10)

AABD-SH should be stored in a cool, dry environment to maintain its chemical stability. It is recommended to keep the reagent in a tightly sealed container to prevent moisture absorption and contamination. Suitable storage containers include glass or high-density polyethylene (HDPE) bottles, which are resistant to chemical interactions. Laboratory personnel should handle the compound with appropriate personal protective equipment (PPE), such as gloves and safety goggles, to ensure safety during use. Proper labeling of storage containers is essential for easy identification and safe handling. Regular inspection of storage conditions ensures the reagent remains effective for analytical applications.

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Analytical Chemistry

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