![TCI A5573 221263-97-4 NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]](http://amiscientific.com/cdn/shop/files/TCI2510A5573_004df647-02bc-437e-8d59-70f43a47b88c.jpg?v=1792658560&width=1946)
![221263-97-4 NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510A5573.jpg?v=1767084917&width=1946)
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NBD-CO-Hz is a chemical compound used in chemical biology, specifically in conjugation chemistry and click chemistry. It serves as a fluorescent labeling agent for molecules in high-performance liquid chromatography (HPLC) analysis. This compound is a derivative of NBD (N-(7-nitrobenzofurazan)), known for its fluorogenic properties. It is widely used in laboratory settings to label and track biological molecules during analytical processes. Its ability to react with amino groups on target molecules makes it a versatile tool for molecular identification and analysis.
NBD-CO-Hz is preferred due to its high reactivity and stability under certain conditions, making it suitable for a variety of chemical and biochemical experiments. It exhibits strong fluorescence, which simplifies the detection and analysis of target molecules through spectroscopy. These properties make it an essential component in fluorescence-based research. The compound's reliability and consistent performance contribute to its popularity among researchers working in biochemistry and analytical chemistry.
In Indonesian laboratories, NBD-CO-Hz is commonly used in biochemical research, pharmacology, and chemical analysis. It plays a key role in studies involving molecular labeling, protein interactions, and the development of molecular detection methods. Its availability and effectiveness make it a valuable resource for scientists conducting experiments that require fluorescent labeling. The compound is essential for advancing research in fields that rely on precise molecular tracking and identification.
Advantages (TCI brochure)
- Reacts with carboxy group in presence of condensing agent to form carbohydrazide
- Carbohydrazide stable for at least one week at 4 C
- Detection limit 2-4 fmol (S/N = 3)
TCI brochures (PDF)
- HPLC Labeling Reagents 2026-03-10 · p. 2
Related TCI products
- TCI A5571 179951-63-4 DBD-CO-Hz [=4-(N,N-Dimethylaminosulfonyl)-7-(N-hydrazinocarbonylmethyl-N-methyl)amino-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5552 33008-06-9 Dansyl Hydrazine [for HPLC Labeling]
- TCI A5576 254973-02-9 AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]
- TCI A5574 189373-41-9 DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5563 143112-51-0 (R)-(-)-NBD-APy [=(R)-(-)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
- TCI A5562 143112-52-1 (S)-(+)-NBD-APy [=(S)-(+)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
- Biochemical Research: NBD-CO-Hz is ideal for labeling biomolecules, enabling detailed analysis of molecular interactions and structural changes.
- Pharmacological Studies: It is used to track drug molecules and their interactions with biological targets, aiding in drug development and mechanism studies.
- HPLC Analysis: The compound serves as a fluorescent label, improving the detection and quantification of compounds in complex mixtures.
- Protein Interaction Studies: Its ability to bind to amino groups allows for the labeling and tracking of proteins in various biochemical assays.
- Molecular Detection Methods: NBD-CO-Hz is employed in the development of sensitive and reliable molecular detection techniques, enhancing analytical accuracy.
From the TCI brochure
- Non-steroidal anti-inflammatory drugs (ketoprofen, flurbiprofen, ibuprofen)
| Brand | TCI |
|---|---|
| CAS number | 221263-97-4 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Chemical Biology > Conjugation Chemistry/Click Chemistry |
| Pack sizes | — |
| Physical form | — |
| Storage | Store in a cool, dry place away from light |
Data from the TCI brochure
| Excitation wavelength | 475 nm (Fluorescence detection) |
|---|---|
| Emission wavelength | 530 nm (Fluorescence detection) |
| Mobile phase | CH3CN / H2O = 70 / 30 (Ambient, Kaseisorb LC ODS 2000, 1 mL/min) |
| Reaction | Carboxylic acid, 20 mM reagent, EDC, pyridine aqueous (DMF-H2O, room temperature, 2 h) |
Source: TCI brochure A1096 (2026-03-10)
NBD-CO-Hz should be stored in a cool, dry environment, away from direct light to maintain its stability and reactivity. It is recommended to keep the compound in a sealed container to prevent moisture absorption and contamination. Proper labeling of storage containers is essential for safe handling and easy identification. Laboratory personnel should wear appropriate personal protective equipment, such as gloves and safety goggles, when handling the compound. The compound should be stored in a well-ventilated area to minimize exposure risks. Regular checks of storage conditions are advised to ensure the compound remains in optimal condition for use.
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Product Categories
- Analytical ReagentsDerivatization ReagentsHPLC Derivatization Reagents
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Carboxy Groups) [HPLC Derivatization Reagents]
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Carbonyl Groups) [HPLC Derivatization Reagents]
Produk Terkait
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