Pembayaran hanya melalui request quotation dan dilakukan setelah tim kami menghubungi Anda. Mohon cantumkan nomor WhatsApp aktif untuk konfirmasi pesanan & pembuatan invoice. Estimasi pengiriman 4-5 minggu setelah pembayaran.
Couldn't load pickup availability
Description
TCI C2255 Chloramphenicol is a broad-spectrum antibiotic that has long been a staple reagent in microbiology and molecular biology laboratories. The compound acts by binding to the bacterial 50S ribosomal subunit and inhibiting peptidyl transferase activity, thereby halting protein synthesis. Beyond antimicrobial testing, chloramphenicol plays a very important role as a selection marker in genetic engineering, because many plasmids and cloning vectors carry a chloramphenicol resistance gene that allows researchers to screen transformed bacterial cells efficiently and reliably.
The characteristic that makes this antibiotic so dependable is its high stability compared with many other antibiotics. Chloramphenicol withstands brief heating, does not readily decompose across the pH range typically used in laboratory work, and its stock solutions in ethanol can be stored for a considerable period without meaningful loss of potency. These properties simplify media preparation and help maintain reproducibility between experiments. Its spectrum of activity covers Gram-positive bacteria, Gram-negative bacteria, and several intracellular organisms, which makes its range of application unusually broad. Chloramphenicol is also bacteriostatic, a character that suits work where cell growth must be arrested rather than the culture destroyed.
In Indonesian laboratories, this reagent is routinely used in academic microbiology teaching, university and institutional research programmes, and molecular biology facilities that perform cloning and transformation work. Its stability is particularly convenient under local working conditions, since prepared stock solutions and supplemented media remain usable across a normal experimental schedule. Laboratories carrying out antimicrobial susceptibility studies, culture maintenance, and plasmid-based selection generally keep chloramphenicol on hand as a standard consumable.
Laboratory Applications
Selection of transformed bacteria: plasmids and cloning vectors carrying a chloramphenicol resistance gene allow efficient screening, so only successfully transformed cells survive on supplemented media.
Antimicrobial susceptibility testing: its broad spectrum against Gram-positive, Gram-negative, and certain intracellular organisms makes it a useful comparative agent in routine microbiological evaluation work.
Preparation of selective culture media: stability toward brief heating and across laboratory pH ranges means the antibiotic survives media preparation without significant loss of activity.
Protein synthesis inhibition studies: by binding the bacterial 50S ribosomal subunit and blocking peptidyl transferase, it provides a well-characterised tool for translation research.
Bacteriostatic growth control: because it arrests bacterial growth rather than killing the culture outright, it suits experiments requiring controlled suppression instead of complete elimination.
Specifications
Brand: TCI
Product code: C2255
CAS number: 56-75-7
Category: Life Science > Biochemicals and Reagents > Antibiotics
Pack sizes: available in the standard catalogue pack sizes offered by TCI for this product
Storage note: store the solid in a closed container in a cool, dry place; ethanol stock solutions may be kept for extended periods without appreciable loss of potency
Handling and Storage
Store chloramphenicol in its original tightly closed container, protected from moisture and kept in a cool, dry area away from direct light and incompatible chemicals. Prepared stock solutions in ethanol should be held in sealed, chemically resistant vials and clearly labelled with the preparation date. Handle the material with gloves, a laboratory coat, and eye protection, and carry out weighing and solution preparation in a well-ventilated area or fume hood to avoid inhaling dust. Use clean, dry spatulas to prevent contamination of the stock, and dispose of residues and contaminated consumables through the laboratory's established chemical and biological waste procedures.
