TCI
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Description
5-Carboxyfluorescein Diacetate (CFDA) is a fluorescent compound widely used in biochemical and biomedical laboratory applications. It serves as a fluorescent indicator that can be activated by specific enzymes or chemical reactions, enabling visual detection or analysis using fluorescence monitoring equipment. This compound plays a critical role in various experimental setups where real-time monitoring of biochemical processes is required. Its ability to respond to environmental changes makes it a versatile tool for studying cellular metabolism and enzyme activity.
CFDA is preferred due to its high stability and sensitivity to chemical changes such as pH and the presence of specific enzymes. These properties allow for precise and reliable detection in complex biological systems. The compound’s fluorescent response is both rapid and consistent, making it ideal for applications requiring high sensitivity and accuracy. Its chemical stability also ensures consistent performance across different experimental conditions, enhancing reproducibility in research settings.
In Indonesian laboratories, CFDA is commonly used in molecular biology, pharmacology, and microbiology research. It supports the development of new analytical methods for cellular and biological process studies. Its reliability and ease of use make it a valuable resource for institutions conducting advanced research in these fields. The compound’s versatility and performance contribute to its widespread adoption in both academic and industrial research environments.
Laboratory Applications
- Molecular biology studies benefit from CFDA’s ability to monitor enzyme activity and cellular metabolism in real time, offering clear fluorescent signals for accurate data collection.
- Pharmacological research utilizes CFDA to assess drug effects on enzyme activity and cellular responses, providing insights into drug mechanisms and efficacy.
- Microbiological investigations employ CFDA to track metabolic changes in microbial cultures, aiding in the study of microbial behavior under varying environmental conditions.
- Fluorescence-based imaging techniques rely on CFDA’s stable and sensitive fluorescent properties to visualize biochemical processes within living cells.
- Environmental monitoring applications use CFDA to detect and quantify specific enzymatic reactions in complex biological samples, enhancing the accuracy of analytical results.
Specifications
- Brand: TCI
- CAS number: 79955-27-4
- Category: Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
- Pack sizes: Available in various quantities as per standard laboratory requirements
- Physical form: Solid powder
- Storage note: Store in a cool, dry place away from direct light
Handling and Storage
CFDA should be stored in a cool, dry place, away from direct light to maintain its stability and fluorescent properties. It is recommended to use airtight containers to prevent moisture absorption and contamination. When handling, ensure proper laboratory safety practices are followed, including the use of gloves and protective eyewear. Avoid exposure to high temperatures or prolonged light exposure, as these can degrade the compound’s effectiveness. Proper storage conditions help preserve the compound’s integrity and ensure consistent performance in experimental applications. Always follow standard laboratory protocols for handling fluorescent compounds to ensure safety and accuracy in research procedures.
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