TCI
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Description
Fluorescein Diacetate (FDA) is a widely used chemical compound in laboratory settings, particularly in the fields of biology and chemistry. It functions as a highly sensitive fluorescent indicator, commonly employed to detect the presence of specific enzymes such as lipase. FDA is known for its ability to accelerate the hydrolysis process, producing fluorescein that is clearly visible under a microscope. This property makes it an essential tool for visualizing enzymatic activity in biological samples. Its versatility and reliability have made it a staple in various laboratory applications, from basic research to advanced biochemical studies.
One of the key reasons FDA is preferred in laboratories is its stable fluorescent properties after hydrolysis. It exhibits fluorescence at specific wavelengths, making it ideal for fluorescence microscopy and spectroscopy techniques. Additionally, FDA is non-toxic and easy to handle, which enhances its safety profile and usability in routine experiments. Its chemical stability under certain conditions ensures long shelf life and consistent performance, reducing the risk of experimental errors. These characteristics contribute to its widespread adoption in both academic and industrial research environments.
In Indonesian laboratories, FDA is extensively used in biomedical, microbiological, and biotechnological research. It plays a crucial role in enzyme activity assays, cellular and tissue monitoring, and fluorescence-based diagnostic techniques. Its relevance in enzyme-related studies makes it a valuable reagent for researchers working on enzyme kinetics, metabolic pathways, and cellular processes. Its ease of use and reliable performance make it a preferred choice for both educational and applied research settings.
Laboratory Applications
- Fluorescence Microscopy: FDA is ideal for visualizing enzymatic activity in cells and tissues, as it fluoresces clearly after hydrolysis by specific enzymes.
- Enzyme Activity Assays: FDA is commonly used to measure the activity of lipases and other hydrolytic enzymes, providing a visual indicator of enzymatic action.
- Biomedical Research: FDA is employed in studies involving cellular metabolism and signal transduction, offering insights into biological processes through fluorescence.
- Microbial Analysis: FDA helps in identifying and studying microbial activity, particularly in assessing the presence of lipolytic bacteria in environmental or clinical samples.
- Cellular Imaging: FDA is used to label and track cellular components, aiding in the visualization of intracellular processes and structural changes.
Specifications
- Brand: TCI
- CAS number: 596-09-8
- Category: Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
- Pack sizes: Available in various quantities as per standard laboratory requirements
- Physical form: Solid powder
- Storage note: Store in a cool, dry place away from direct sunlight
Handling and Storage
FDA should be stored in a cool, dry place away from direct sunlight to maintain its stability and fluorescence properties. It is recommended to use airtight containers to prevent moisture absorption, which could affect its performance. Due to its non-toxic nature, handling is generally safe, but standard laboratory precautions should still be followed, such as wearing gloves and safety goggles when working with powdered reagents. FDA does not require special storage conditions beyond those typical for most biochemical reagents, making it easy to manage in a standard laboratory setting. Proper storage ensures consistent results and prolongs the shelf life of the product.
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