Fluorescein 6-Isothiocyanate (isomer II) TCI F0783

Name: TCI F0783 18861-78-4 Fluorescein 6-Isothiocyanate (isomer II)
Brand: TCI
SKU: TCI2510F078330747
Price: 9591750.0 IDR
Availability: InStock

Rp 9.591.750,00

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Berat 100mg

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TCI F0783 Fluorescein 6-Isothiocyanate (isomer II) is a fluorescent labelling reagent widely known in molecular biology and biochemistry laboratories, commonly referred to as FITC. The compound consists of a fluorescein core that emits bright green fluorescence when excited by blue light, combined with an isothiocyanate group that acts as a reactive anchor toward amine functionalities. Its role in the laboratory is to link an easily detected optical signal to biological molecules such as proteins, antibodies, peptides, or oligosaccharides, so that the presence and distribution of those molecules can be observed directly.

The characteristics that make this reagent a preferred choice are the combination of strong fluorescence output and selective labelling reactivity. The isothiocyanate group reacts with primary amines, particularly free amino groups at chain termini as well as on lysine side chains, forming a thiourea linkage that is covalently stable and not readily lost during washing steps. Offering this product as a single isomer, namely isomer II, is an important advantage, because labelling with an isomer mixture can produce heterogeneous conjugates and complicate reproducibility between batches. A single isomer supports more consistent conjugation chemistry and more comparable results from one preparation to the next.

In Indonesian laboratories, this reagent is typically used in university research groups, biochemistry and molecular biology teaching laboratories, and analytical service units that prepare their own fluorescent conjugates rather than purchasing ready-made labelled reagents. It is drawn on where fluorescence microscopy, flow cytometry, or fluorescence-based detection is part of the routine workflow, and where glycoscience and chemical biology studies require biological molecules to be tagged with a visible optical reporter before observation.

Protein and antibody labelling, because the isothiocyanate group couples covalently to primary amines on lysine residues and chain termini, producing stable fluorescent conjugates that survive repeated washing.
Fluorescence microscopy of cells and tissues, since the fluorescein core emits bright green fluorescence under blue-light excitation and is readily visualised with standard filter configurations used for FITC.
Flow cytometry sample preparation, where labelled antibodies and binding proteins give a strong optical signal that allows individual cells to be distinguished and counted reliably.
Glycoscience research on oligosaccharides and glycoconjugates, in which amine-bearing sugar derivatives are tagged so that their presence and distribution can be tracked during separation and detection.
Peptide labelling for binding and localisation studies, because the single-isomer format gives more homogeneous conjugates and improves reproducibility between preparations compared with isomer mixtures.

Brand	TCI
CAS number	18861-78-4
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Glycoscience
Pack sizes	available in the pack sizes listed on this product page
Physical form	—
Storage	store under the conditions stated on the manufacturer's label, protected from light

Product code: F0783

Store the reagent in its original tightly closed container, protected from light, since fluorescein derivatives are light sensitive and the isothiocyanate group is reactive toward moisture and amines. Amber glass vials or containers wrapped in foil are suitable, and the container should be allowed to reach room temperature before opening to limit condensation. Handle the solid in a well-ventilated area or fume hood while wearing gloves, safety glasses, and a laboratory coat, and avoid contact with skin, eyes, and inhalation of dust. Prepare working solutions fresh where possible, keep them shielded from light during use, and consult the manufacturer's safety data sheet before handling and for waste disposal.

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