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Description
TCI F0810 Fluorescein-5-maleimide is a fluorescent labeling reagent designed specifically for tagging thiol groups on proteins, peptides, and other biomolecules. The molecule combines fluorescein, one of the most classical and widely used fluorophores in the life sciences, with a maleimide group that reacts selectively with free sulfhydryl groups. The Michael addition reaction between the maleimide and a thiol proceeds rapidly under near-neutral conditions and produces a stable thioether bond, allowing researchers to attach a fluorescent marker to specific cysteine residues without disturbing amine groups or other functional groups on the biomolecule.
The advantages that make this reagent a frequent choice are the selectivity of its reaction and the brightness of its fluorophore. Thiol selectivity permits labeling at a defined position, an essential requirement in studies of protein structure, measurements of distance between residues, and monitoring of conformational change. Fluorescein itself has a high absorption coefficient and strong green emission within an excitation range available on nearly every fluorescence microscope, microplate reader, and flow cytometer, so that specialised instrumentation is not required to detect the labeled product.
In Indonesian laboratories, this reagent is typically used in university and institutional research settings where protein chemistry, biochemistry, and molecular biology work is carried out. Because fluorescein is detectable on the standard fluorescence instrumentation already present in most local facilities, the reagent fits readily into existing workflows for protein characterisation, conjugate preparation, and fluorescence-based assay development without additional equipment investment.
Laboratory Applications
- Site-specific protein labeling: the maleimide group reacts selectively with free cysteine residues, allowing a fluorescent tag to be placed at one defined position on the protein without modifying amine groups.
- Protein structure studies: because labeling occurs at a defined residue, the attached fluorescein serves as a reporter for investigating structural organisation and the spatial arrangement of a protein of interest.
- Distance measurement between residues: defined-position labeling supports experiments that determine the separation between selected residues, where the exact placement of the fluorophore is critical to interpretation.
- Conformational change monitoring: a fluorescein tag attached at a specific cysteine reports changes in the local environment, making it suitable for following conformational transitions in proteins under different conditions.
- Fluorescence detection workflows: the high absorption coefficient and strong green emission of fluorescein allow labeled products to be read on ordinary fluorescence microscopes, microplate readers, and flow cytometers.
Specifications
- Brand: TCI
- CAS number: 75350-46-8
- Category: Life Science > Antibodies > Antibody Supplementary Products
- Product name: Fluorescein-5-maleimide (contains 2% N,N-Dimethylformamide at maximum)
- Pack sizes: please refer to the available packaging options listed for this catalogue item
- Storage: store according to the conditions stated on the manufacturer's label and product documentation
Handling and Storage
Store the reagent in its original tightly closed container under the conditions specified on the manufacturer's label, protected from light, since fluorescein-based fluorophores are sensitive to photodegradation and prolonged light exposure may reduce labeling performance. Keep the container in a dry location and allow it to reach room temperature before opening to limit moisture condensation, which can hydrolyse the maleimide group. Handle the material in a well-ventilated area or fume hood, using gloves, safety glasses, and a laboratory coat, and note that the product contains a maximum of 2% N,N-Dimethylformamide. Consult the safety data sheet supplied by the manufacturer before use, and dispose of residues in accordance with applicable laboratory waste procedures.
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