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TCI

CAS 134471-24-2

N-Succinimidyl 7-Hydroxycoumarin-3-carboxylate TCI S0866

N-Succinimidyl 7-Hydroxycoumarin-3-carboxylate TCI S0866

Chemical Identity

CAS No.
134471-24-2
PubChem
CID 5706554·SID 135727308
Formula
C14H9NO7
Molecular weight
303.22 g/mol
Purity
>96.0%(HPLC)(T)
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
(2,5-dioxopyrrolidin-1-yl) 7-hydroxy-2-oxochromene-3-carboxylate
SMILES
C1CC(=O)N(C1=O)OC(=O)C2=CC3=C(C=C(C=C3)O)OC2=O
InChIKey
KFEBWCYYRFZMTJ-UHFFFAOYSA-N
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N-Succinimidyl 7-Hydroxycoumarin-3-carboxylate is a chemical compound widely used in biochemical and molecular biology applications. It is a key reagent in laboratory settings, particularly for activating and conjugating molecules such as antigens and antibodies. Its unique structure allows for covalent bonding with proteins and other biomolecules, making it an essential tool in biomedical research. This compound is frequently employed in labeling and detection processes, enabling researchers to track and analyze complex biological systems with high specificity.

The compound's chemical properties make it a preferred choice for various experimental protocols. It exhibits stability under controlled conditions, ensuring reliable performance in laboratory procedures. Its reactivity can be modulated, allowing researchers to fine-tune activation processes for optimal results. The compound's ability to form covalent bonds with a wide range of substrates enhances its versatility, making it suitable for multiple applications. Its consistent performance and accuracy contribute to the reliability of experimental outcomes.

In Indonesian laboratories, N-Succinimidyl 7-Hydroxycoumarin-3-carboxylate is commonly used in molecular biology, immunology, and biomedical research. It supports studies involving protein labeling, antibody conjugation, and fluorescence-based detection methods. Its role in these fields is critical for advancing scientific understanding and developing new diagnostic and therapeutic approaches. The compound's reliability and effectiveness make it a valuable asset in both academic and industrial research environments.

Advantages (TCI brochure)

  • Blue fluorescence
  • Fluorescence spectra depend on pH

TCI brochures (PDF)

  • Molecular Biology Research – This compound is ideal for labeling proteins and nucleic acids, enabling precise tracking and analysis of biological molecules.
  • Immunological Studies – It is used to conjugate antibodies, enhancing their detection capabilities in immunoassays and fluorescence-based techniques.
  • Biomedical Diagnostics – Its ability to form stable covalent bonds makes it suitable for developing sensitive and specific diagnostic tools.
  • Fluorescence Imaging – The compound's fluorescent properties allow for the visualization of biomolecules in complex biological systems.
  • Protein Conjugation – It is widely used to attach functional groups to proteins, facilitating various biochemical and analytical applications.

From the TCI brochure

  • Can be labeled to amino groups

Brand TCI
CAS number 134471-24-2
Molecular formula —
Purity —
Category Life Science > Antibodies > Antibody Supplementary Products
Pack sizes —
Physical form —
Storage Store at controlled room temperature, away from moisture and light

Data from the TCI brochure

λex,max342 nm (pH4 buffer)
λem,max447 nm (pH4 buffer)

Source: TCI brochure LL039 (2025-11-18)

This compound should be stored at controlled room temperature, away from moisture and direct light to maintain its stability and effectiveness. It is recommended to keep it in a sealed container to prevent exposure to air and humidity, which could affect its chemical integrity. Proper labeling of storage containers is essential to ensure safe handling and prevent accidental contamination. Laboratory personnel should use appropriate personal protective equipment, such as gloves and safety goggles, when handling the compound. It is important to follow standard laboratory safety protocols to minimize risks associated with chemical exposure. Regular monitoring of storage conditions is advised to ensure the compound remains in optimal condition for use in research applications.

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