5-Carboxyfluorescein N-Succinimidyl Ester TCI C2479

Chemical Identity

CAS No.: 92557-80-7
PubChem: CID 2762599·SID 253659702
Formula: C25H15NO9
Molecular weight: 473.39 g/mol
Purity: >90.0%(HPLC)
Storage: <0°C
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
SMILES: C1CC(=O)N(C1=O)OC(=O)C2=CC3=C(C=C2)C4(C5=C(C=C(C=C5)O)OC6=C4C=CC(=C6)O)OC3=O
InChIKey: GECIDMICWWDIBO-UHFFFAOYSA-N
MDL: MDL00077322

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5-Carboxyfluorescein N-Succinimidyl Ester (CFSE) is a fluorescent compound widely used in biomedical research and molecular biology. It serves as a vital tool for tracking cell proliferation, particularly in immunology and cancer biology studies. This reagent enables researchers to label and monitor the division of cells over time, offering valuable insights into cellular behavior and response mechanisms. Its application is critical in experiments that require precise and reproducible data, making it an essential component in modern laboratory workflows.

CFSE is preferred due to its chemical stability, high reactivity toward cell surface amines, and distinct emission wavelength. These properties ensure reliable fluorescence detection using instruments like flow cytometers, enhancing the accuracy of cell tracking and analysis. Its strong fluorescence and resistance to photobleaching allow for long-term studies without significant loss of signal intensity. Additionally, its rapid reaction kinetics and ability to maintain fluorescence after activation make it highly practical for repeated use in various experimental setups.

In Indonesian laboratories, CFSE is commonly used in molecular biology and immunology research. It supports studies on immune cell dynamics, tumor progression, and cellular responses to stimuli. Researchers in Indonesia rely on CFSE to generate high-quality data for both academic and applied research, contributing to advancements in biotechnology and life sciences. Its utility spans across different research institutions, ensuring its relevance in the scientific community.

Advantages (TCI brochure)

Green fluorescence
Labels amino groups without a coupling agent due to active NHS ester

TCI brochures (PDF)

Iodinated Fluorescein for Various Applications 2024-11-22 · p. 2
Fluorescent Labeling Dyes 2025-11-18 · p. 3
Reagents for Biological Research 2025-03-11 · p. 5

Related TCI products

Cell proliferation studies benefit from CFSE due to its ability to label and track cell division over time, providing accurate data on cell cycle dynamics.
Immunology research utilizes CFSE to monitor immune cell activation and differentiation, helping to understand immune responses in various disease models.
Flow cytometry applications rely on CFSE for its distinct fluorescence properties, enabling precise quantification of labeled cells in complex mixtures.
Cancer biology experiments use CFSE to study tumor cell growth and response to therapeutic agents, offering insights into treatment efficacy.
Molecular biology labs apply CFSE in tracking cell lineage and migration, supporting research on cellular behavior under different experimental conditions.

From the TCI brochure

Labeling of amino groups

Brand	TCI
CAS number	92557-80-7
Molecular formula	—
Purity	—
Category	Life Science > Antibodies > Antibody Supplementary Products
Pack sizes	Available in various quantities as per supplier specifications
Physical form	Solid or powder, depending on packaging
Storage	Store at 2–8°C in a cool, dry place away from light

Data from the TCI brochure

λex,max	494 nm
λem,max	521 nm

Source: TCI brochure LL039 (2025-11-18)

CFSE should be stored at 2–8°C in a cool, dry place, away from direct light to maintain its chemical stability and fluorescence properties. It is recommended to use airtight containers to prevent moisture exposure, which could affect its reactivity. When handling, ensure proper laboratory safety practices are followed, including the use of gloves and protective eyewear. Avoid prolonged exposure to heat or UV light to prevent degradation. CFSE is generally stable under standard laboratory conditions but should be used promptly after opening to ensure optimal performance. Always follow standard operating procedures for handling fluorescent reagents to maintain data integrity and safety.

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