TCI
5-Carboxyfluorescein N-Succinimidyl Ester TCI C2479
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Description
5-Carboxyfluorescein N-Succinimidyl Ester (CFSE) is a fluorescent compound widely used in biomedical research and molecular biology. It serves as a vital tool for tracking cell proliferation, particularly in immunology and cancer biology studies. This reagent enables researchers to label and monitor the division of cells over time, offering valuable insights into cellular behavior and response mechanisms. Its application is critical in experiments that require precise and reproducible data, making it an essential component in modern laboratory workflows.
CFSE is preferred due to its chemical stability, high reactivity toward cell surface amines, and distinct emission wavelength. These properties ensure reliable fluorescence detection using instruments like flow cytometers, enhancing the accuracy of cell tracking and analysis. Its strong fluorescence and resistance to photobleaching allow for long-term studies without significant loss of signal intensity. Additionally, its rapid reaction kinetics and ability to maintain fluorescence after activation make it highly practical for repeated use in various experimental setups.
In Indonesian laboratories, CFSE is commonly used in molecular biology and immunology research. It supports studies on immune cell dynamics, tumor progression, and cellular responses to stimuli. Researchers in Indonesia rely on CFSE to generate high-quality data for both academic and applied research, contributing to advancements in biotechnology and life sciences. Its utility spans across different research institutions, ensuring its relevance in the scientific community.
Laboratory Applications
- Cell proliferation studies benefit from CFSE due to its ability to label and track cell division over time, providing accurate data on cell cycle dynamics.
- Immunology research utilizes CFSE to monitor immune cell activation and differentiation, helping to understand immune responses in various disease models.
- Flow cytometry applications rely on CFSE for its distinct fluorescence properties, enabling precise quantification of labeled cells in complex mixtures.
- Cancer biology experiments use CFSE to study tumor cell growth and response to therapeutic agents, offering insights into treatment efficacy.
- Molecular biology labs apply CFSE in tracking cell lineage and migration, supporting research on cellular behavior under different experimental conditions.
Specifications
- Brand: TCI
- CAS number: 92557-80-7
- Category: Life Science > Antibodies > Antibody Supplementary Products
- Pack sizes: Available in various quantities as per supplier specifications
- Physical form: Solid or powder, depending on packaging
- Storage note: Store at 2–8°C in a cool, dry place away from light
Handling and Storage
CFSE should be stored at 2–8°C in a cool, dry place, away from direct light to maintain its chemical stability and fluorescence properties. It is recommended to use airtight containers to prevent moisture exposure, which could affect its reactivity. When handling, ensure proper laboratory safety practices are followed, including the use of gloves and protective eyewear. Avoid prolonged exposure to heat or UV light to prevent degradation. CFSE is generally stable under standard laboratory conditions but should be used promptly after opening to ensure optimal performance. Always follow standard operating procedures for handling fluorescent reagents to maintain data integrity and safety.
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