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Description
5-Carboxyfluorescein (CF) is a fluorescent compound widely used in biochemical and molecular biology experiments. It serves as a vital tool in laboratory settings for detecting and analyzing biological processes such as protein activity, cellular behavior, and molecular conformation changes. Its ability to emit light under specific conditions makes it an essential component in various analytical techniques. CF is commonly employed in flow cytometry and fluorescence microscopy to track cell populations and monitor biochemical reactions in real time.
The chemical stability and controlled reactivity of CF make it a preferred choice for researchers. Its solubility in organic solvents simplifies its use in a variety of experimental conditions. The solid form of CF allows for safe storage and easy dilution when required. These properties ensure that CF remains a reliable and versatile reagent in both routine and advanced laboratory applications.
In Indonesian laboratories, 5-Carboxyfluorescein is extensively used in cell biology, immunology, and pharmacology research. It supports studies involving fluorescent labeling, enzyme activity assays, and cellular imaging. Its relevance in these fields makes it an indispensable tool for scientists seeking to enhance the accuracy and efficiency of their experiments.
Laboratory Applications
Flow Cytometry: CF is used to label and track cell populations, enabling the analysis of cell surface markers and intracellular components.
Fluorescence Microscopy: CF provides high-contrast imaging for studying cellular structures and molecular interactions under a microscope.
Enzyme Activity Assays: CF serves as a fluorescent substrate to monitor enzyme activity and reaction kinetics in biochemical experiments.
Cellular Imaging: CF is employed to visualize dynamic processes such as protein translocation and membrane dynamics in living cells.
Immunofluorescence Studies: CF is utilized to label and detect specific proteins in immunological research, aiding in the identification of target antigens.
Specifications
Brand: TCI
CAS number: 76823-03-5
Category: Life Science > Antibodies > Antibody Supplementary Products
Pack sizes: Available in various quantities as per standard laboratory requirements
Physical form: Solid
Storage note: Store in a cool, dry place away from light
Handling and Storage
5-Carboxyfluorescein should be stored in a cool, dry place away from direct light to maintain its stability and fluorescence properties. It is recommended to use airtight containers to prevent moisture absorption and contamination. When handling CF, it is important to follow standard laboratory safety protocols, including the use of gloves and protective eyewear. Avoid exposure to high temperatures or prolonged light exposure to preserve its chemical integrity. Proper labeling of containers ensures safe handling and reduces the risk of accidental misuse. Always ensure that the compound is used in well-ventilated areas to minimize any potential health risks.
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