6-Carboxyfluorescein TCI C2478

Chemical Identity

Other names: 6-FAM · 6-FAM
CAS No.: 3301-79-9
PubChem: CID 76806·SID 125307953
Formula: C21H12O7
Molecular weight: 376.32 g/mol
Purity: >97.0%(HPLC)
Reference databases: ChEBI·ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
SMILES: C1=CC2=C(C=C1C(=O)O)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O
InChIKey: BZTDTCNHAFUJOG-UHFFFAOYSA-N
MDL: MDL00036873

Rp 4.611.600,00

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6-Carboxyfluorescein (CF) is a fluorescent compound widely used in biomedical and research applications. It is commonly employed as a fluorescent marker in laboratories for identifying and tracking specific cells or molecules. Its ability to emit blue light when exposed to green light makes it an essential tool in fluorescence-based experiments. This material is particularly valuable in cellular imaging and molecular analysis due to its high sensitivity and specificity. CF is also used in conjugation processes to label antibodies, enhancing detection capabilities in various diagnostic and research settings.

The chemical properties of CF make it a preferred choice for laboratory use. It exhibits stability under controlled laboratory conditions and is non-toxic, ensuring safe handling. CF demonstrates good resistance to variations in pH and temperature, which simplifies storage and application across different experimental environments. Additionally, it enhances fluorescent signals without interfering with the biological activity of the molecules being studied. These characteristics contribute to its reliability and effectiveness in a wide range of research applications.

In Indonesian laboratories, 6-Carboxyfluorescein is extensively used in biomedical, clinical, and academic research settings. It is a key component in experiments related to cell tracking, molecular analysis, and antibody labeling. Its versatility and performance make it a popular choice among researchers in Indonesia, supporting both basic and applied scientific investigations. The compound's consistent performance and ease of use further solidify its role in the research community.

Advantages (TCI brochure)

Green fluorescence
Labeled to amino groups by amide bond formation using a coupling agent

TCI brochures (PDF)

Iodinated Fluorescein for Various Applications 2024-11-22 · p. 2
Fluorescent Labeling Dyes 2025-11-18 · p. 3
Reagents for Biological Research 2025-03-11 · p. 5

Related TCI products

Fluorescence Imaging: CF is ideal for fluorescence imaging due to its bright and stable emission, enabling clear visualization of cellular structures and molecular interactions.
Antibody Labeling: CF is used to label antibodies for enhanced detection in immunological assays, providing high sensitivity and specificity in diagnostic applications.
Cell Tracking: Its ability to emit light under specific wavelengths makes it suitable for tracking cell movement and behavior in live-cell experiments.
Molecular Analysis: CF is widely used in molecular analysis techniques such as flow cytometry and fluorescence spectroscopy for detecting and quantifying biomolecules.
Diagnostic Testing: CF's fluorescent properties are applied in diagnostic testing to identify and monitor biological markers in clinical and research settings.

From the TCI brochure

Labeling of amino groups

Brand	TCI
CAS number	3301-79-9
Molecular formula	—
Purity	—
Category	Life Science > Antibodies > Antibody Supplementary Products
Pack sizes	—
Physical form	—
Storage	Store in a cool, dry place away from light

Data from the TCI brochure

λex,max	494 nm
λem,max	521 nm

Source: TCI brochure LL039 (2025-11-18)

6-Carboxyfluorescein should be stored in a cool, dry place away from direct light to maintain its stability and fluorescence properties. It is recommended to use airtight containers to prevent contamination and moisture exposure. While it is non-toxic, standard laboratory safety precautions should be followed when handling the compound. Avoid prolonged exposure to high temperatures or extreme pH conditions to preserve its integrity. Proper labeling and storage ensure its effectiveness in research applications. Regular inspection of storage conditions is advised to ensure optimal performance in laboratory experiments.

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