2',7'-Dichlorodihydrofluorescein Diacetate TCI D4409

Name: TCI D4409 4091-99-0 2',7'-Dichlorodihydrofluorescein Diacetate
Brand: TCI
SKU: TCI2510D440924990
Price: 2982000.0 IDR
Availability: InStock

Rp 2.982.000,00

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Berat 100mg

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Description

2',7'-Dichlorodihydrofluorescein Diacetate (DCFH-DA) is a fluorescent probe widely used in life sciences laboratories for detecting reactive oxygen species (ROS) in biological systems. This compound serves as a sensitive indicator for oxidative stress, allowing researchers to monitor cellular responses to oxidative challenges. Its ability to fluoresce upon oxidation makes it a valuable tool in biochemical and biomedical research. DCFH-DA is commonly employed in experiments that require the visualization and quantification of free radical activity within cells, making it an essential reagent for studying oxidative damage and cellular signaling pathways.

DCFH-DA is preferred due to its high sensitivity, non-toxic nature, and ease of use. It exhibits excellent fluorescence intensity, enabling precise detection of even low levels of oxidative stress. The compound is stable under appropriate storage conditions and remains compatible with a wide range of cell types and experimental setups. Its fluorescent properties are highly responsive to environmental changes within cells, offering reliable and reproducible results. These characteristics make DCFH-DA a go-to choice for researchers seeking accurate and consistent data in their studies.

In Indonesian laboratories, DCFH-DA is frequently used in biochemistry and biomedical research settings. It is commonly found in studies related to oxidative stress, cell signaling, and disease mechanisms. Researchers in Indonesia rely on this compound for its reliability, versatility, and compatibility with various experimental protocols. Its application spans from basic research to applied studies, supporting a wide array of scientific investigations in both academic and industrial settings.

Laboratory Applications

Oxidative Stress Analysis - DCFH-DA is ideal for measuring reactive oxygen species in cells, offering a reliable method to assess oxidative damage and cellular responses.
Cellular Signaling Studies - Its fluorescent properties allow researchers to visualize and quantify oxidative stress markers, aiding in the understanding of intracellular signaling pathways.
Immunological Research - DCFH-DA helps in studying immune cell activation and oxidative stress responses, providing insights into inflammatory and autoimmune conditions.
Toxicology Testing - The compound is used to evaluate the effects of environmental toxins and drugs on cellular oxidative status, supporting safety and efficacy assessments.
Disease Mechanism Investigations - DCFH-DA enables the study of oxidative stress in various diseases, including neurodegenerative disorders and metabolic syndromes.

Specifications

Brand: TCI
CAS Number: 4091-99-0
Category: Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Pack Sizes: Available in various quantities as per supplier specifications
Physical Form: Solid powder
Storage Note: Store at 2-8°C in a dry, light-protected environment

Handling and Storage

DCFH-DA should be stored in a cool, dry place away from direct light to maintain its stability and fluorescence properties. It is recommended to keep the compound in a tightly sealed container to prevent moisture absorption and contamination. Due to its non-toxic nature, it is generally safe to handle, but standard laboratory safety protocols should still be followed. When working with DCFH-DA, it is important to use appropriate personal protective equipment, such as gloves and safety goggles, to ensure safe handling. The compound is compatible with most common laboratory solvents and reagents, making it versatile for various experimental applications. Proper storage and handling ensure the integrity and effectiveness of DCFH-DA in research settings.