2',7'-Dichlorofluorescein [for Fluorescent indicator] TCI D0371

Chemical Identity

CAS No.: 76-54-0
PubChem: CID 64944·SID 87567058
Formula: C20H10Cl2O5
Molecular weight: 401.20 g/mol
Purity: >95.0%(HPLC)
Reference databases: ChEBI·ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
SMILES: C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C=C4OC5=CC(=C(C=C35)Cl)O)O)Cl
InChIKey: VFNKZQNIXUFLBC-UHFFFAOYSA-N
MDL: MDL00005047

Rp 955.500,00

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2',7'-Dichlorofluorescein is a fluorescent compound widely used as a fluorescent indicator in chemical and biological experiments. It is a key reagent in life science laboratories, where it plays a crucial role in detecting specific ions and monitoring chemical reactions through fluorescence. This compound is particularly valuable for its ability to emit light under certain conditions, making it an essential tool for both qualitative and quantitative analysis. Its application spans various fields, including biochemistry, analytical chemistry, and environmental science, where precise detection and measurement are required.

The compound's high sensitivity to environmental changes and its ability to emit light within a specific spectral range make it a preferred choice for fluorescence-based techniques. These properties enable researchers to track molecular interactions and monitor cellular processes with high accuracy. Additionally, its stability under controlled conditions ensures reliable results across multiple experiments. This combination of sensitivity, specificity, and stability makes 2',7'-Dichlorofluorescein a versatile and reliable reagent in modern laboratory settings.

In Indonesian laboratories, 2',7'-Dichlorofluorescein is extensively used in biochemistry, analytical chemistry, and environmental research. It supports academic institutions, research centers, and industrial laboratories by providing a cost-effective and efficient means of conducting fluorescence-based experiments. Its widespread use highlights its importance in advancing scientific research and educational programs in Indonesia.

TCI brochures (PDF)

Iodinated Fluorescein for Various Applications 2024-11-22 · p. 1
Titration Reagents and TLC Stains 2026-05-07 · p. 10

Related TCI products

Fluorescence Microscopy: This item is ideal for observing fluorescently labeled cells and tissues, allowing detailed analysis of cellular structures and processes.
Flow Cytometry: It is used to detect and quantify fluorescent markers in cells, enabling the study of cell populations and their characteristics.
Metal Ion Detection: The compound's ability to react with metal ions makes it suitable for identifying heavy metals in environmental and biological samples.
Environmental Monitoring: It is employed in assessing water and soil quality by detecting contaminants through fluorescence response.
Biochemical Assays: It serves as a fluorescent indicator in various biochemical reactions, providing real-time data on reaction progress and outcomes.

Brand	TCI
CAS number	76-54-0
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Pack sizes	Available in various quantities as per standard laboratory requirements
Physical form	Solid powder
Storage	Store in a cool, dark place away from moisture and light

Data from the TCI brochure

λex, max	511 nm
λem, max	533 nm

Source: TCI brochure FF197 (2024-11-22)

This compound should be stored in a cool, dark environment to maintain its stability and prevent degradation. It is recommended to use airtight containers to protect it from moisture and light exposure. Proper labeling of containers is essential for safe handling and identification. Laboratory personnel should wear appropriate personal protective equipment when handling the compound to ensure safety. The compound should be kept away from incompatible substances to avoid any chemical reactions. Regular monitoring of storage conditions ensures the compound remains effective for experimental use.

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