Phenolphthalin [for redox indicator] from TCI (product code P0095) is the reduced form of phenolphthalein. Its structure is almost the same as phenolphthalein's, but it lacks the conjugated system that produces colour, so the compound itself is colourless. When oxidised, phenolphthalin converts back to phenolphthalein, which turns pink under alkaline conditions. Analytical laboratories therefore use it as a redox indicator to detect oxidising agents or peroxidase-like catalytic activity in a sample.
Laboratories choose this reagent because its colour change is very distinct: it goes from colourless to bright pink, which is easy to see by eye without special instruments. Haem groups, together with hydrogen peroxide, can catalyse its oxidation. This principle underlies the presumptive blood test known as the Kastle-Meyer test. TCI supplies this phenolphthalin specifically for redox indicator work, so its quality suits analytical tasks that need a clear and repeatable colour response from one run to the next.
In Indonesia, phenolphthalin is useful for analytical chemistry, forensic, biochemistry and chemistry education laboratories. Lecturers and laboratory staff can use it to demonstrate redox reactions and the link between chemical structure and colour in practical classes. Forensic and research laboratories can use it for presumptive screening that relies on peroxidase-like activity. AMI Scientific supplies this TCI reagent to universities, research institutions and testing laboratories that need a dependable titration and indicator reagent for routine and educational work.
Related TCI products
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- TCI A5008 14979-11-4 Dimethylsulfonazo III [Spectrophotometric reagent for alkaline earth metals and indicator for the precipitation titration of SO4 with Ba]
- TCI D0371 76-54-0 2',7'-Dichlorofluorescein [for Fluorescent indicator]
- TCI A5107 62637-91-6 TBPE (=Tetrabromophenolphthalein Ethyl Ester Potassium Salt) [Sensitive spectrophotometric reagent for amines, quaternary ammonium salts and other cations]
- TCI P0099 518-51-4 Phenolphthalein Disodium Salt (Water soluble)
- Redox indicator work: the colourless reduced form turns into pink phenolphthalein when oxidised, which gives a clear visual signal that an oxidising agent is present in the tested solution.
- Kastle-Meyer presumptive blood testing: haem groups and hydrogen peroxide catalyse the oxidation of phenolphthalin, so this reagent is the basis of this well-known forensic screening method.
- Detecting peroxidase-like catalytic activity: the reaction responds to catalytic oxidation, so biochemistry laboratories can use it to show peroxidase-like behaviour in samples or model systems.
- Chemistry education and practical classes: the sharp change from colourless to bright pink lets lecturers show students redox chemistry and the role of conjugated systems in colour.
- Routine analytical checks that need a visual endpoint: the colour response is clear and repeatable, so analysts can judge results by eye without spectrophotometers or other special instruments.
| Brand | TCI (product code P0095) |
|---|---|
| CAS number | 81-90-3 |
| Molecular formula | — |
| Purity | — |
| Category | Analytical Chemistry > Analytical Reagents > Titration Reagents |
| Pack sizes | see the pack sizes listed on the product page |
| Physical form | refer to the TCI product label and Safety Data Sheet (SDS) |
| Storage | — |
- Grade/intended use: for redox indicator
Keep phenolphthalin in its original, tightly closed container in a cool, dry, well-ventilated place, away from direct sunlight and heat. Because the compound turns coloured when oxidised, keep it away from oxidising agents and peroxides so that it does not degrade or give false positive results. Label the container clearly and keep it away from incompatible chemicals. Handle it following good laboratory practice: wear gloves, safety glasses and a lab coat, avoid creating or breathing in dust, and wash your hands after use. Always check the TCI Safety Data Sheet for specific storage temperatures, hazards and disposal instructions before use.
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