Fluorescein Chloride [Reagent for Amines] TCI D0369

Chemical Identity

Other names: 3',6'-Dichlorofluoran
CAS No.: 630-88-6
PubChem: CID 99508·SID 87567057
Formula: C20H10Cl2O3
Molecular weight: 369.20 g/mol
Purity: >95.0%(HPLC)
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 3',6'-dichlorospiro[2-benzofuran-3,9'-xanthene]-1-one
SMILES: C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)Cl)OC5=C3C=CC(=C5)Cl
InChIKey: LNBKJWCRFAZVLK-UHFFFAOYSA-N
MDL: MDL00037841

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Fluorescein Chloride [Reagent for Amines] from TCI is a fluorescein derivative in which both hydroxyl groups on the xanthene skeleton have been replaced by chlorine atoms. TCI classifies this product as an analytical reagent for spectroscopy, specifically as a reagent for amines. In the laboratory, the compound acts as a precursor or reagent that reacts with amines to form coloured or fluorescent xanthene derivatives. These derivatives can then be observed and measured by spectrophotometric or fluorometric techniques.

Researchers choose this compound because the xanthene skeleton is the basis of many important fluorescent dyes, such as fluorescein and rhodamine, which are known for strong absorbance and bright fluorescence. The chlorine atoms sit at the positions normally occupied by hydroxyl groups, and amines can displace them. This reaction gives a route to amino-xanthene dyes with tunable optical properties. In its lactone form, the compound is generally not strongly coloured, so the change in signal after reaction can be used for detection.

In Indonesian laboratories, Fluorescein Chloride is relevant to analytical chemistry researchers who develop amine detection methods. University research groups working on dye synthesis and fluorescent probes can use it as a starting material for xanthene-based compounds. Spectroscopy laboratories can use it to study how amine substitution changes absorbance and fluorescence. Teaching laboratories in chemistry departments may also use it to show how a colourless precursor turns into a coloured or fluorescent product.

Related TCI products

Amine detection: the compound reacts with amines to form coloured or fluorescent xanthene derivatives, which can be measured by spectrophotometry or fluorometry.
Synthesis of amino-xanthene dyes: amines can displace the chlorine atoms, giving a practical route to rhodamine-type dyes with tunable optical properties.
Fluorescent probe development: the weakly coloured lactone form changes signal after reaction, which suits researchers designing turn-on detection systems for amine-containing analytes.
Spectroscopic method development: analytical chemists can use the reaction products to build and validate absorbance and fluorescence measurement methods based on the xanthene chromophore.
Chemistry education and research training: the visible change from a weakly coloured precursor to a coloured or fluorescent product clearly shows structure–property relationships in xanthene dyes.

Brand	TCI (product code D0369)
CAS number	630-88-6
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Spectroscopy Reagents
Pack sizes	as listed on the product page; contact AMI Scientific to confirm availability
Physical form	—
Storage	follow the conditions stated on the TCI label and Safety Data Sheet

Store Fluorescein Chloride in its original tightly closed container, in a cool, dry, well-ventilated place away from direct light and moisture. Keep it separate from strong oxidizing agents and from amines or other reactive nucleophiles, so the reagent is not consumed before use. Always check the TCI label and Safety Data Sheet for specific storage temperatures and hazard information. When handling, wear suitable gloves, safety glasses and a laboratory coat, and work in a fume hood or well-ventilated area. Avoid creating dust, avoid contact with skin and eyes, and do not inhale the material. Close the container right after use, label any transferred portions clearly, and dispose of waste according to institutional and local chemical waste regulations.

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