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Description
5-Chlorosaligenyl-N,N-diisopropylphosphoramidite is a chemical compound widely used in chemical biology, particularly in nucleic acid chemistry. It serves as a key precursor in the synthesis of phosphoramidite nucleotides, which are essential components in the production of oligonucleotides. This compound plays a crucial role in molecular biology laboratories where the construction of DNA or RNA strands is required for various experimental applications. Its chemical structure enables specific reactions under controlled conditions, making it a versatile tool in modern biochemical research.
The compound is valued for its reactivity and stability under specific conditions. Its ability to selectively bind to nucleotide bases ensures accuracy and precision in nucleic acid synthesis. This makes it particularly suitable for applications requiring high fidelity, such as DNA sequencing or probe design. The compound’s stability also allows for reliable performance in laboratory settings, contributing to the reproducibility of experimental results. These properties make it a preferred choice for researchers working in nucleic acid chemistry.
In Indonesian laboratories, 5-Chlorosaligenyl-N,N-diisopropylphosphoramidite is commonly used by researchers in universities, research centers, and biotechnology labs. It supports studies in genetics, biomedicine, and chemical biology, which are rapidly growing fields in Indonesia. Its application is essential for advancing research in molecular biology and related disciplines, providing a foundation for innovative scientific discoveries.
Laboratory Applications
DNA sequencing relies on this compound to synthesize oligonucleotide probes with high specificity and accuracy.
Oligonucleotide synthesis benefits from its reactivity, enabling the precise construction of DNA and RNA strands.
PCR experiments use this compound to create primers that ensure accurate amplification of target DNA sequences.
Probe design in molecular biology requires its selective binding properties to generate reliable hybridization results.
Genetic research utilizes this compound to develop custom nucleic acid sequences for functional and structural studies.
Specifications
Brand: TCI
CAS number: 1620086-77-2
Category: Chemistry > Chemical Biology > Nucleic Acid Chemistry
Pack sizes: Not specified
Physical form: Not specified
Storage note: Store in a cool, dry place away from light
Handling and Storage
This compound should be stored in a cool, dry environment, away from direct light to maintain its stability and reactivity. It is recommended to use airtight containers to prevent exposure to moisture and air, which could affect its chemical integrity. Due to its chemical nature, it should be handled in a well-ventilated area, and appropriate personal protective equipment such as gloves and safety goggles should be worn. Avoid contact with skin and eyes, and ensure that spill containment measures are in place. Proper disposal should follow local regulations to ensure safety and environmental compliance.
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