TCI
2'-Deoxycytidine 5'-Monophosphate Hydrate TCI D3673
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Description
TCI D3673 2'-Deoxycytidine 5'-Monophosphate Hydrate, commonly abbreviated as dCMP, is a deoxyribose nucleotide composed of a cytosine base, a 2'-deoxyribose sugar, and a single phosphate group at the 5' position. This compound is one of the fundamental building blocks of DNA, which gives it an important position as a biochemical reagent. In the laboratory, dCMP is used as a substrate or reference product in studies of nucleotide metabolism, as a comparison material in nucleic acid composition analysis, and as a starting point for the synthesis of nucleotide derivatives.
The hydrate form indicates the presence of water molecules bound within the crystal, a point that must be taken into account when calculating molar mass for the preparation of solutions at precise concentrations. The properties that make this reagent a frequent choice are its good solubility in aqueous media, adequate stability as a solid when stored cold and dry, and a clearly defined chemical identity that makes it suitable as a working analytical standard. The phosphate group imparts acidic character and a negative charge that is exploited in separations by ion-exchange chromatography as well as ion-pair reversed-phase liquid chromatography. The cytosine base has a characteristic ultraviolet absorption.
In Indonesian laboratories, this reagent is typically used in academic and research settings where nucleotide chemistry and nucleic acid analysis are routine. Its role as a defined reference material supports method development and verification work in chromatographic analysis, while its function as a DNA building block makes it relevant to biochemistry teaching and to research groups examining nucleotide metabolism. Laboratories that prepare nucleotide derivatives also draw on it as a synthetic starting material.
Laboratory Applications
- Nucleotide metabolism studies — dCMP serves as a substrate or reference product in metabolic investigations because it is a defined, well-characterized deoxyribonucleotide with a clearly established chemical identity.
- Nucleic acid composition analysis — the compound acts as a comparison material against which sample components are identified, supported by the characteristic ultraviolet absorption of its cytosine base.
- Synthesis of nucleotide derivatives — the molecule provides a defined starting point for preparative chemistry, offering a reactive 5' phosphate group and a deoxyribose sugar for further modification work.
- Ion-exchange chromatography — the acidic phosphate group carries a negative charge that allows retention and separation on ion-exchange columns, making dCMP suitable as a chromatographic reference.
- Ion-pair reversed-phase liquid chromatography — the same negatively charged phosphate group is exploited in ion-pair separations, so this reagent works well as a working standard for such analytical methods.
Specifications
- Brand: TCI
- CAS number: 1032-65-1
- Category: Life Science > Biochemicals and Reagents > Nucleosides, Nucleotides, Oligonucleotides
- Physical form: solid, supplied as the hydrate with water molecules bound in the crystal
- Pack sizes: available in the pack sizes offered by TCI for this catalogue item
- Storage: keep cold and dry to maintain stability of the solid
Handling and Storage
Store this reagent cold and dry, since the solid shows adequate stability under these conditions. Keep the material in its original tightly closed container, or in a comparable well-sealed chemical container that limits exposure to atmospheric moisture, and return it to cold storage promptly after weighing. Because the product is a hydrate, allow the container to reach room temperature before opening so that condensation does not affect the mass taken, and remember to account for the bound water when calculating molar mass for precise solution preparation. Handle in a clean laboratory area using gloves, eye protection, and a laboratory coat, and use clean, dry spatulas to avoid contaminating the stock.
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