Dodecyldimethyl(3-sulfopropyl)ammonium Hydroxide Inner Salt [for Biochemical Research] TCI D3860

Name: TCI D3860 14933-08-5 Dodecyldimethyl(3-sulfopropyl)ammonium Hydroxide Inner Salt [for Biochemical Research]
Brand: TCI
SKU: TCI2510D386024259
Price: 856800.0 IDR
Availability: InStock

Rp 856.800,00

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Berat 5g 25g

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Description

TCI D3860 Dodecyldimethyl(3-sulfopropyl)ammonium Hydroxide Inner Salt is a zwitterionic surfactant of the sulfobetaine class, prepared for biochemical research use. Its molecule carries a dual-charged head group — a positively charged quaternary ammonium centre and a negatively charged sulfonate group held within a single structure — paired with a hydrophobic dodecyl hydrocarbon tail. This arrangement allows the compound to act as a detergent capable of solubilising membrane proteins and disrupting lipid structures, while remaining net neutral in overall charge across the biological working pH range commonly used in the laboratory.

The zwitterionic character of this material is the principal reason it is selected over alternatives. Unlike strongly ionic detergents such as SDS, which impose excess charge on proteins, sulfobetaines solubilise and denature more gently, so many proteins can still be analysed according to their native charge. This property matters a great deal in two-dimensional electrophoresis, isoelectric focusing, and proteomic sample preparation. In addition, zwitterionic surfactants generally do not interfere with ion exchange chromatography, because they do not bind to charged resin, which gives the analyst greater freedom in designing a purification workflow.

In Indonesian laboratories, this reagent is typically used in university research groups, biotechnology institutes, and protein and proteomics laboratories where membrane protein extraction and charge-based separation are routine. It is normally purchased in research-scale quantities for method development and repeat sample preparation rather than for production use, and is handled alongside buffers, chaotropic agents, and other detergents as part of a standard protein solubilisation and electrophoresis sample workflow.

Laboratory Applications

Membrane protein solubilisation: the hydrophobic dodecyl tail inserts into lipid structures and disrupts them, releasing integral membrane proteins into solution without the excess charge loading caused by strongly ionic detergents.
Two-dimensional electrophoresis sample preparation: because the surfactant is net neutral at biological working pH, proteins retain their native charge and can be resolved correctly in the charge-based first dimension.
Isoelectric focusing: the zwitterionic head group does not add net charge to the protein, so isoelectric points are not artificially shifted and focusing behaviour reflects the true protein character.
Proteomic sample preparation: gentle solubilisation and denaturation keep a wide range of proteins in an analysable state, supporting reproducible extraction from complex biological samples before downstream separation.
Ion exchange chromatography workflows: zwitterionic surfactants generally do not bind to charged resin, so this detergent can remain in the sample without interfering with column behaviour or purification design.

Specifications

Brand: TCI (Tokyo Chemical Industry)
CAS number: 14933-08-5
Catalogue code: D3860
Category: Life Science > Biochemicals and Reagents > Denaturation
Grade: for biochemical research use
Pack sizes: available in standard research-scale catalogue pack sizes; please confirm the current pack options when ordering
Storage: store according to the manufacturer's label and accompanying documentation

Handling and Storage

Store the container tightly closed in a cool, dry place away from direct sunlight, moisture, and incompatible chemicals, following the storage condition stated on the manufacturer's label and safety data sheet. Keep the material in its original supplier container where possible, or in a clean, clearly labelled, chemically compatible and well-sealed container if transferred. Handle in a well-ventilated area or fume hood using a laboratory coat, safety goggles, and suitable gloves, and avoid generating dust or aerosols when weighing. Use clean, dry spatulas and glassware to prevent contamination or moisture uptake, close the container immediately after use, and wash hands thoroughly after handling. Dispose of residues and contaminated materials in accordance with applicable institutional and local laboratory waste regulations.