Hexadecyldimethyl(3-sulfopropyl)ammonium Hydroxide Inner Salt [for Biochemical Research] TCI H1283

Hexadecyldimethyl(3-sulfopropyl)ammonium Hydroxide Inner Salt is a zwitterionic surfactant of the sulfobetaine class. Its structure combines one long hexadecyl alkyl chain, a permanently positively charged quaternary ammonium centre, and a negatively charged sulfonate group at the end of a propyl arm. Because both charges reside within the same molecule, the compound carries no net charge across a wide pH range. Designated as a material for biochemical research, it is used as a membrane-solubilising detergent, as a dispersing agent, and as a surface ligand in colloidal quantum dot research.

The zwitterionic character is precisely why this reagent is chosen. Unlike ionic detergents such as SDS, which readily denature proteins, long-chain sulfobetaines are able to solubilise membrane proteins while leaving their structure and biological activity comparatively intact. The compound also does not interfere with charge-based separations such as isoelectric focusing or two-dimensional electrophoresis, and it remains stable over broad ranges of pH and ionic strength. In materials work, the long C16 chain provides effective steric stabilisation at nanocrystal surfaces while the zwitterionic head group keeps the colloid well dispersed.

In Indonesian laboratories, this reagent is typically found in university and institutional research groups working on membrane protein biochemistry, proteomics sample preparation, and electronic or nanomaterials development. It suits laboratories that run protein extraction and two-dimensional gel workflows, as well as groups preparing colloidal quantum dot dispersions for optoelectronic studies. Because it is supplied as a research-grade material, it is normally handled within established laboratory protocols rather than in production settings.

• Membrane protein solubilisation: the long-chain sulfobetaine disrupts lipid bilayers and extracts integral membrane proteins while preserving their folded structure and biological activity far better than harsh ionic detergents.
• Two-dimensional gel electrophoresis sample preparation: because the molecule carries no net charge, it solubilises proteins without distorting the charge-based first dimension of the separation.
• Isoelectric focusing: the zwitterionic head group does not contribute to the sample's charge state, so proteins migrate to their true isoelectric points without detergent-induced artefacts.
• Colloidal quantum dot surface ligand studies: the C16 chain supplies steric stabilisation at the nanocrystal surface while the zwitterionic head maintains colloidal dispersion in research-scale quantum dot preparations.
• Dispersing agent for nanomaterials: the combined steric and zwitterionic stabilisation keeps particles suspended across a broad window of pH and ionic strength typical of materials science experiments.

• Grade designation: for biochemical research

Store the material in its original tightly closed container, kept dry and protected from moisture, in a cool location away from direct sunlight and heat sources. Surfactants of this type are best kept in a dedicated chemical cabinet, with the container reclosed promptly after each use to limit moisture uptake. Handle in a well-ventilated area or fume hood, wearing a laboratory coat, safety glasses, and chemically resistant gloves, and avoid generating dust, inhalation, and contact with skin or eyes. Use clean, dry spatulas and glassware when weighing, label all prepared solutions clearly, and dispose of residues and contaminated materials in accordance with local laboratory waste procedures. Always consult the manufacturer's safety data sheet before first use.

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