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Description
TCI D4292 is 5,6-Dichlorobenzimidazole 1-beta-D-Ribofuranoside, a nucleoside analogue that combines a chlorinated benzimidazole base with a ribofuranose sugar in the beta configuration. The compound is widely known among molecular biology researchers as a reagent for interfering with transcription in cells, and it is commonly abbreviated as DRB in the literature. As a material in the biochemicals and reagents category, this product is intended for cell-based experiments and gene expression analysis rather than for large-scale chemical synthesis work.
The feature that makes this compound a preferred choice is its ability to enter cells and act as an inhibitor of the kinase associated with RNA chain elongation by RNA polymerase II, so that its effect on transcription can be observed within a relatively short time frame and is reversible once the reagent is washed out. Its nucleoside structure gives it reasonable solubility in polar organic solvents such as dimethyl sulfoxide, making it straightforward to prepare as a concentrated stock solution that is then diluted into culture medium. The presence of two chlorine atoms on the benzimidazole ring adds lipophilic character that assists cell membrane penetration compared with more polar nucleosides.
In Indonesian laboratories, this reagent is typically used in molecular biology and cell biology settings where transcription needs to be modulated under controlled conditions. It is handled at the bench scale of cell culture and gene expression work, prepared as a stock solution in a polar organic solvent and then applied to culture medium at working concentrations. Its rapid and reversible action suits experimental designs that compare cells before, during, and after treatment within a single working session.
Laboratory Applications
Transcription inhibition studies: the compound enters cells readily and interferes with the transcription process, allowing researchers to establish a defined transcriptional shutdown point in cultured cells.
RNA polymerase II elongation research: because it inhibits the kinase associated with RNA chain elongation by RNA polymerase II, it is well suited to work examining the elongation phase specifically rather than general transcription.
Gene expression analysis: the reagent supports experiments where transcript levels must be measured following a controlled interruption of transcription, making expression changes easier to interpret over time.
Reversible treatment experiments: its effect can be reversed after the reagent is washed out, which suits designs comparing treated and recovered cell populations within the same experimental series.
Cell-based reagent screening: its lipophilic chlorinated benzimidazole ring assists membrane penetration, so it performs well in live-cell assays that require the compound to reach intracellular targets quickly.
Specifications
Brand: TCI
CAS Number: 53-85-0
Chemical name: 5,6-Dichlorobenzimidazole 1-beta-D-Ribofuranoside
Category: Life Science > Biochemicals and Reagents > Nucleosides, Nucleotides, Oligonucleotides
Pack sizes: available in standard TCI research-scale packaging; please confirm the available pack size when ordering
Storage: store according to the manufacturer's stated conditions on the product label
Handling and Storage
Store the product in its original tightly closed container, kept in a cool, dry place and protected from light and moisture, following the storage conditions stated by the manufacturer on the product label. Stock solutions prepared in dimethyl sulfoxide should be kept in sealed, chemically compatible vials and clearly labelled with the preparation date and concentration. Handle the material in a well-ventilated area or a fume hood, wearing gloves, safety glasses, and a laboratory coat, and avoid the generation of dust when weighing. Keep the container away from incompatible materials, use clean and dry spatulas to prevent contamination of the bulk material, and consult the manufacturer's safety data sheet before use and before disposal of any residues.
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