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Description
Ethyl 2-Methylvalerate is a branched ester formed from 2-methylvaleric acid and ethanol, in which a methyl group is attached to the alpha carbon immediately adjacent to the carbonyl group. This branching position is precisely what makes the small molecule scientifically interesting, because that alpha carbon constitutes a stereogenic centre. In the laboratory the material serves as a model compound in biochemical studies of lipids and esters, as an enzyme assay substrate, and as a chromatographic reference standard in the analysis of volatile components in food samples and natural products.
The characteristics behind its selection include a liquid form that is easily measured volumetrically, good solubility in organic solvents, and a volatility well suited to gas chromatographic analysis. The branching at the alpha position imposes a distinctive steric hindrance, so that its rate of enzymatic hydrolysis differs markedly from that of linear esters; this very difference is exploited to assess the specificity of lipases and esterases. Because the compound is racemic at the alpha carbon, it also makes an appropriate substrate for enzymatic kinetic resolution experiments that yield enrichment of one enantiomer.
In the Indonesian laboratory setting, this reagent is typically used in biochemistry and enzymology work, in food and natural-product volatile profiling, and in teaching and research activities where a well-defined branched ester is required as a reference or model substrate. It is supplied under the TCI brand within the Life Science category, under Biochemicals and Reagents, in the Lipids group, and is handled as a volatile organic liquid using ordinary laboratory practice.
Laboratory Applications
- Lipase specificity screening: the alpha-branched structure creates steric hindrance that alters enzymatic hydrolysis rates relative to linear esters, revealing how selective a given lipase truly is.
- Esterase activity assays: the compound acts as a defined ester substrate whose cleavage can be followed to characterise esterase behaviour under controlled biochemical assay conditions.
- Enzymatic kinetic resolution studies: because the alpha carbon is a stereogenic centre and the material is racemic, it suits experiments that enrich one enantiomer preferentially.
- Gas chromatography reference standard: its suitable volatility and liquid form make it a convenient comparison peak when identifying volatile ester components in analytical runs.
- Volatile profiling of food and natural products: it serves as a branched-ester reference for confirming the identity of comparable volatile constituents in such sample matrices.
Specifications
- Brand: TCI
- CAS number: 39255-32-8
- Category: Life Science > Biochemicals and Reagents > Lipids
- Pack sizes: available in standard laboratory catalogue pack sizes; confirm the required size when ordering.
- Physical form: liquid, easily measured volumetrically, soluble in organic solvents.
- Storage: keep in a tightly closed container in a cool, well-ventilated place away from ignition sources.
Handling and Storage
Store the material in its original tightly closed container in a cool, dry, well-ventilated area away from heat, sparks, and open flame, since it is a volatile organic liquid. Chemically compatible glass bottles with solvent-resistant closures are appropriate, and containers should be reclosed promptly after dispensing to limit evaporation and moisture uptake. Handle within a fume hood or under adequate ventilation, wear safety glasses, gloves, and a laboratory coat, and avoid contact with skin, eyes, and clothing. Transfer volumetrically with clean, dry glassware, keep the working area free of incompatible materials, and follow local institutional procedures for labelling, spill response, and chemical waste disposal.
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