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Description
DL-Histidine is the racemic form of the amino acid histidine, consisting of an equal mixture of the D and L enantiomers and carrying the characteristic imidazole side chain. It belongs to the group of semi-essential amino acids and participates in protein synthesis, in the enzymatic formation of histamine, and in the regulation of acid-base balance within the cell. In the TCI catalogue this product is listed under the code H0148 and is positioned within the peptide chemistry product line. In the laboratory, DL-Histidine is used as a raw material for growth media, as a component of biological buffers, as a metal-chelating reagent, and as a comparison compound in studies of enzyme selectivity toward stereochemical configuration.
The characteristic that makes this compound a frequent choice is the presence of the imidazole ring, whose pKa value lies close to physiological pH. Because of this, histidine is able to accept and release protons across the pH range at which biological systems operate, allowing it to act as an effective natural buffering species. The same imidazole group also confers the ability to bind transition metal ions such as nickel, copper, and zinc, a property that forms the basis of various protein purification techniques founded on metal affinity. As a racemic form, DL-Histidine is generally more economical than the pure L form.
In Indonesian laboratories, DL-Histidine is typically encountered in biochemistry, microbiology, and molecular biology work. It is drawn on when growth media must be prepared or supplemented, when buffer systems need a component that is active near physiological pH, and when metal-binding behaviour is being examined. Its role as a reference compound in stereoselectivity work also makes it useful in teaching laboratories and in research groups that compare enzyme responses toward the D and L configurations of the same amino acid.
Laboratory Applications
- Preparation of microbial growth media: DL-Histidine serves as an amino acid raw material that supplies the nitrogen and carbon skeleton required for culture formulation and supplementation work.
- Formulation of biological buffer systems: the imidazole side chain has a pKa close to physiological pH, so the compound buffers effectively across the working range of biological experiments.
- Metal chelation reagent: the imidazole group binds transition metal ions such as nickel, copper, and zinc, making the compound useful wherever controlled metal coordination is required.
- Metal-affinity protein purification studies: the same imidazole-metal interaction underlies affinity-based purification techniques, so this amino acid supports method development and elution experiments.
- Enzyme stereoselectivity comparison: as a racemate containing both D and L enantiomers, it acts as a reference material when testing how enzymes discriminate between stereochemical configurations.
Specifications
- Brand: TCI
- CAS number: 4998-57-6
- Catalogue code: H0148
- Category: Chemistry > Chemical Biology > Peptide Chemistry
- Pack sizes: available in the standard catalogue pack sizes offered for this item; please confirm the required quantity when ordering
- Storage: store in a closed original container under the conditions stated on the product label
Handling and Storage
Store DL-Histidine in its original tightly closed container, kept in a cool, dry, and well-ventilated area away from moisture and direct sunlight, and follow any storage condition printed on the product label. Suitable containers include the supplied packaging or a clean, well-sealed glass or chemically compatible plastic vessel with a clear label. Handle the material in a clean working area, use standard laboratory protective equipment such as gloves, safety glasses, and a laboratory coat, and avoid generating or inhaling dust. Use clean, dry spatulas when weighing to prevent contamination of the remaining stock, close the container immediately after use, and consult the manufacturer safety data sheet before handling.
