2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid, commonly known as HEPES, is a vital chemical compound used as a buffer in various biological experiments. It plays a crucial role in maintaining the pH stability of solutions, which is essential for accurate and reproducible results in biochemical and biomedical research. HEPES is particularly effective in buffering solutions within a specific pH range, making it indispensable for experiments that require precise pH control. Its widespread use in laboratory settings highlights its importance in supporting reliable scientific investigations.
HEPES is preferred due to its stability, non-toxicity, and non-volatility. These properties make it a safe and reliable choice for use in biological research environments. Its ability to resist pH changes caused by chemical reactions or biological processes ensures consistent experimental conditions. Additionally, HEPES is compatible with a wide range of biological materials, including cells, proteins, and enzymes, which enhances its versatility in laboratory applications. Its chemical inertness also minimizes interference with experimental outcomes, ensuring accurate data collection.
In Indonesian laboratories, HEPES is widely used in research related to cell biology, enzymology, and protein studies. It is a preferred choice due to its availability, high quality, and consistent performance across various experimental setups. Researchers in educational and research institutions rely on HEPES as a fundamental component for experiments requiring precise pH control. Its reliability and effectiveness have made it a standard in both academic and industrial research settings.
- Cell culture media preparation, as HEPES helps maintain optimal pH for cell growth and viability.
- Enzymatic reaction buffers, where HEPES ensures stable pH conditions for enzymatic activity and substrate interaction.
- Protein purification processes, as HEPES provides a stable environment for protein folding and solubility.
- Electrophoresis buffer solutions, where HEPES supports consistent pH levels for accurate separation of biomolecules.
- Tissue culture experiments, as HEPES maintains physiological pH levels necessary for cell survival and function.
| Brand | TCI |
|---|---|
| CAS number | 7365-45-9 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Biological Buffers |
| Pack sizes | Available in various quantities as per supplier specifications |
| Physical form | Solid or powder, depending on packaging |
| Storage | Store in a cool, dry place away from light and moisture |
HEPES should be stored in a cool, dry environment, away from direct sunlight and moisture to maintain its chemical integrity. It is recommended to use airtight containers to prevent exposure to humidity and potential contamination. Due to its non-volatile nature, HEPES does not evaporate easily, but proper sealing is still essential for long-term storage. Handling should be done with appropriate personal protective equipment, such as gloves and safety goggles, to ensure safety during preparation and use. It is important to avoid contact with skin or eyes and to work in a well-ventilated area to minimize any potential risks. Regular inspection of storage conditions ensures the product remains effective for its intended applications.
—
![2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research] (CAS 7365-45-9) - TCI H0396 - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510H0396.jpg?v=1767116343&width=1445)
