Piperazine-1,4-bis(2-ethanesulfonic Acid) Monosodium Salt is a chemical compound widely used as a buffer in biological research applications. It plays a crucial role in maintaining stable pH levels in various experimental setups, ensuring optimal conditions for biochemical reactions. This buffer is particularly valuable in experiments where precise pH control is essential for the integrity of biological samples and enzymatic activity. Its ability to resist pH changes makes it an essential component in many laboratory protocols, especially in life sciences research.
This compound is preferred due to its chemical stability and excellent buffering capacity within a specific pH range. It is highly soluble in water, making it easy to incorporate into different types of solutions. Its non-toxic nature and safe handling profile further enhance its appeal for use in laboratory environments. These properties collectively make it a reliable and versatile choice for researchers working in biochemistry and biomedicine.
In Indonesian laboratories, this buffer is commonly used in a variety of research contexts, including protein analysis, enzyme studies, and cellular experiments. Its ability to maintain a stable pH environment is critical for the accuracy and reproducibility of experimental results. It is also favored for its compatibility with a wide range of analytical techniques, making it a staple in many research settings across the country.
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- Used in biomedical research to maintain pH stability in biochemical reaction mixtures, ensuring consistent experimental outcomes.
- Suitable for enzyme activity assays where precise pH control is necessary to mimic physiological conditions.
- Applied in protein purification processes to preserve the structural integrity of proteins during chromatography and electrophoresis.
- Employed in cellular studies to create stable pH environments for cell culture and biochemical assays.
- Ideal for buffer preparation in analytical techniques such as HPLC and electrophoresis, where pH consistency is critical.
| Brand | TCI |
|---|---|
| CAS number | 10010-67-0 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Biological Buffers |
| Pack sizes | Available in various quantities as per supplier specifications |
| Physical form | Solid powder |
| Storage | Store in a cool, dry place away from moisture and direct sunlight |
This material should be stored in a cool, dry place, away from moisture and direct sunlight to maintain its chemical stability. It is recommended to use airtight containers to prevent exposure to humidity, which could affect its buffering capacity. As a non-toxic substance, it is generally safe to handle, but standard laboratory precautions should still be followed, such as wearing gloves and safety goggles. It is not flammable or reactive under normal conditions, making it suitable for routine use in most laboratory environments. Proper labeling and storage are essential to ensure safe handling and prevent cross-contamination.
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